語系:
繁體中文
English
說明(常見問題)
回圖書館首頁
手機版館藏查詢
登入
回首頁
切換:
標籤
|
MARC模式
|
ISBD
Molecular insights into the transcri...
~
Lee, Min Gyu X.
FindBook
Google Book
Amazon
博客來
Molecular insights into the transcriptional regulation of the type II hexokinase gene in highly glycolytic hepatoma cells and hepatocytes.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Molecular insights into the transcriptional regulation of the type II hexokinase gene in highly glycolytic hepatoma cells and hepatocytes./
作者:
Lee, Min Gyu X.
面頁冊數:
134 p.
附註:
Source: Dissertation Abstracts International, Volume: 64-10, Section: B, page: 4775.
Contained By:
Dissertation Abstracts International64-10B.
標題:
Biology, Molecular. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3107533
ISBN:
0496550828
Molecular insights into the transcriptional regulation of the type II hexokinase gene in highly glycolytic hepatoma cells and hepatocytes.
Lee, Min Gyu X.
Molecular insights into the transcriptional regulation of the type II hexokinase gene in highly glycolytic hepatoma cells and hepatocytes.
- 134 p.
Source: Dissertation Abstracts International, Volume: 64-10, Section: B, page: 4775.
Thesis (Ph.D.)--The Johns Hopkins University, 2004.
One of the most common biochemical phenotypes of highly malignant, rapidly growing tumors is their ability to utilize glucose at high rates dependent on hexokinase, the first enzyme of the glycolytic pathway. In such tumors, hexokinase is markedly elevated (>100 fold) and plays an essential role in sustaining the high glycolytic phenotype. Among the four mammalian hexokinase types (HK I--IV), type II (HKII) is predominantly overexpressed in such tumors. In contrast, HKII expression is nearly silent in many normal cells, e.g. hepatocytes. Molecular mechanisms involved in the differential regulation of the HKII gene have not been well understood. Using AS-30D hepatoma cells as a highly glycolytic cancer cell model, work described here has shown that the enhanced activity of the HKII promoter resides within a short segment (-281 to -35) of the proximal region and that the transcription factors Sp1, Sp2, Sp3, NF-Y, and CREB interact with sites in this region. In addition, the transcription factors Sp1, Sp2, Sp3, NF-Y, and CREB appear to reside together in a complex bound to the proximal promoter region thereby activating HKII expression. In contrast to hepatoma cells, the levels of transcription factors Sp1, Sp2, Sp3, NF-Y, and CREB are very low in primary hepatocytes and the binding of these factors to the HKII promoter is not detectable under the conditions of the assay employed. In summary, these studies provide novel insights into how the HKII gene may be differentially regulated in normal and cancer cells. Finally, in an independent but related study, it has been shown that the transcription factor Mnt/Rox is induced by a pH decrease in the medium.
ISBN: 0496550828Subjects--Topical Terms:
1017719
Biology, Molecular.
Molecular insights into the transcriptional regulation of the type II hexokinase gene in highly glycolytic hepatoma cells and hepatocytes.
LDR
:02676nmm 2200301 4500
001
1837522
005
20050506072709.5
008
130614s2004 eng d
020
$a
0496550828
035
$a
(UnM)AAI3107533
035
$a
AAI3107533
040
$a
UnM
$c
UnM
100
1
$a
Lee, Min Gyu X.
$3
1925968
245
1 0
$a
Molecular insights into the transcriptional regulation of the type II hexokinase gene in highly glycolytic hepatoma cells and hepatocytes.
300
$a
134 p.
500
$a
Source: Dissertation Abstracts International, Volume: 64-10, Section: B, page: 4775.
500
$a
Adviser: Peter L. Pedersen.
502
$a
Thesis (Ph.D.)--The Johns Hopkins University, 2004.
520
$a
One of the most common biochemical phenotypes of highly malignant, rapidly growing tumors is their ability to utilize glucose at high rates dependent on hexokinase, the first enzyme of the glycolytic pathway. In such tumors, hexokinase is markedly elevated (>100 fold) and plays an essential role in sustaining the high glycolytic phenotype. Among the four mammalian hexokinase types (HK I--IV), type II (HKII) is predominantly overexpressed in such tumors. In contrast, HKII expression is nearly silent in many normal cells, e.g. hepatocytes. Molecular mechanisms involved in the differential regulation of the HKII gene have not been well understood. Using AS-30D hepatoma cells as a highly glycolytic cancer cell model, work described here has shown that the enhanced activity of the HKII promoter resides within a short segment (-281 to -35) of the proximal region and that the transcription factors Sp1, Sp2, Sp3, NF-Y, and CREB interact with sites in this region. In addition, the transcription factors Sp1, Sp2, Sp3, NF-Y, and CREB appear to reside together in a complex bound to the proximal promoter region thereby activating HKII expression. In contrast to hepatoma cells, the levels of transcription factors Sp1, Sp2, Sp3, NF-Y, and CREB are very low in primary hepatocytes and the binding of these factors to the HKII promoter is not detectable under the conditions of the assay employed. In summary, these studies provide novel insights into how the HKII gene may be differentially regulated in normal and cancer cells. Finally, in an independent but related study, it has been shown that the transcription factor Mnt/Rox is induced by a pH decrease in the medium.
590
$a
School code: 0098.
650
4
$a
Biology, Molecular.
$3
1017719
650
4
$a
Chemistry, Biochemistry.
$3
1017722
650
4
$a
Biology, Cell.
$3
1017686
650
4
$a
Health Sciences, Oncology.
$3
1018566
690
$a
0307
690
$a
0487
690
$a
0379
690
$a
0992
710
2 0
$a
The Johns Hopkins University.
$3
1017431
773
0
$t
Dissertation Abstracts International
$g
64-10B.
790
1 0
$a
Pedersen, Peter L.,
$e
advisor
790
$a
0098
791
$a
Ph.D.
792
$a
2004
856
4 0
$u
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3107533
筆 0 讀者評論
館藏地:
全部
電子資源
出版年:
卷號:
館藏
1 筆 • 頁數 1 •
1
條碼號
典藏地名稱
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
W9187036
電子資源
11.線上閱覽_V
電子書
EB
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
評論
新增評論
分享你的心得
Export
取書館
處理中
...
變更密碼
登入