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A novel resistive pulse sensor for b...

Saleh, Omar Adel.

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A novel resistive pulse sensor for biological measurements.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	A novel resistive pulse sensor for biological measurements./
作者:	Saleh, Omar Adel.
面頁冊數:	111 p.
附註:	Source: Dissertation Abstracts International, Volume: 63-10, Section: B, page: 4546.
Contained By:	Dissertation Abstracts International63-10B.
標題:	Biophysics, General. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3067018
ISBN:	049386492X

A novel resistive pulse sensor for biological measurements.
Saleh, Omar Adel.

A novel resistive pulse sensor for biological measurements. - 111 p.

Source: Dissertation Abstracts International, Volume: 63-10, Section: B, page: 4546.

Thesis (Ph.D.)--Princeton University, 2003.

Resistive pulse analysis is an experimental technique that allows measurements of the sizes of solution-bound particles. It functions by measuring the electrical resistance of a pore that connects two solution-filled reservoirs: a particle entering the pore displaces conducting fluid, which leads to a transient change (or pulse) in the measured resistance. The magnitude and duration of the pulse are related to the size and position of the particle that caused it.

ISBN: 049386492XSubjects--Topical Terms:

1019105
Biophysics, General.

A novel resistive pulse sensor for biological measurements.
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100 1 $a Saleh, Omar Adel. $3 1925908
245 1 2 $a A novel resistive pulse sensor for biological measurements.
300 $a 111 p.
500 $a Source: Dissertation Abstracts International, Volume: 63-10, Section: B, page: 4546.
500 $a Adviser: Lydia Sohn.
502 $a Thesis (Ph.D.)--Princeton University, 2003.
520 $a Resistive pulse analysis is an experimental technique that allows measurements of the sizes of solution-bound particles. It functions by measuring the electrical resistance of a pore that connects two solution-filled reservoirs: a particle entering the pore displaces conducting fluid, which leads to a transient change (or pulse) in the measured resistance. The magnitude and duration of the pulse are related to the size and position of the particle that caused it.
520 $a We have developed a novel platform for performing resistive pulse analysis on a microfabricated device containing a pore that is between 0.2 and 1 mum in diameter. We use the device to measure latex colloids with diameters between 100 and 600 nm, and to measure single macromolecules of DNA. We quantify the relation between the particle geometry and the pulse shape, and we find that particles traveling off of the pore axis significantly affect the measured pulses in a manner dependent on the force used to drive the particles through the pore (electrophoresis or fluid flow). We have developed a novel algorithm that increases the precision of measurements using fluid flow by removing these off-axis effects. This algorithm is not specific to our device, and should be useful for all future applications of the resistive pulse technique.
520 $a With this improved precision, we are able to measure nanometer-scale changes in the diameters of protein-coated colloids when they specifically bind to free protein in the solution---thus demonstrating the first successful strategy to add analyte specificity to a solid-state resistive pulse sensor. We find the measured diameter to be a useful probe of free protein concentration in the range 0.5 to 5 mug/mL; future versions of the device should make it sensitive to concentrations as low as 1 ng/mL. We also demonstrate the applicability of this technique to performing common immunological assays, where small antigens in the solution are detected by the binding (or lack thereof) of antibody to the colloids.
590 $a School code: 0181.
650 4 $a Biophysics, General. $3 1019105
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710 2 0 $a Princeton University. $3 645579
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790 1 0 $a Sohn, Lydia, $e advisor
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791 $a Ph.D.
792 $a 2003
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