東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Development of solid phase microextr...

Lord, Heather Lynn.

FindBook

Google Book

Amazon

博客來

Development of solid phase microextraction devices for in vivo analysis.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Development of solid phase microextraction devices for in vivo analysis./
作者:	Lord, Heather Lynn.
面頁冊數:	338 p.
附註:	Source: Dissertation Abstracts International, Volume: 67-08, Section: B, page: 4391.
Contained By:	Dissertation Abstracts International67-08B.
標題:	Health Sciences, Toxicology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NR17462
ISBN:	9780494174623

Development of solid phase microextraction devices for in vivo analysis.
Lord, Heather Lynn.

Development of solid phase microextraction devices for in vivo analysis. - 338 p.

Source: Dissertation Abstracts International, Volume: 67-08, Section: B, page: 4391.

Thesis (Ph.D.)--University of Waterloo (Canada), 2006.

The quest for better drug analysis may be defined in many ways, depending on the needs of the analyst. A method may be characterized by its sensitivity, specificity, accuracy, reproducibility, robustness, speed, or cost. Depending on the particular problem at hand, one characteristic may be valued above another. Given the foregoing, the overriding goal of the research presented was to develop a novel probe for in vivo monitoring of drug concentrations. The anticipated benefits were in terms of selectivity, speed of analysis due to a simplification of the sample preparation process, as well as the ability to monitor therapeutically relevant (free) drug concentrations in a dynamic living system.

ISBN: 9780494174623Subjects--Topical Terms:

1017752
Health Sciences, Toxicology.

Development of solid phase microextraction devices for in vivo analysis.
LDR:03219nmm 2200301 4500 001 1831495
005 20070511144450.5
008 130610s2006 eng d
020 $a 9780494174623
035 $a (UnM)AAINR17462
035 $a AAINR17462
040 $a UnM $c UnM
100 1 $a Lord, Heather Lynn. $3 1920279
245 1 0 $a Development of solid phase microextraction devices for in vivo analysis.
300 $a 338 p.
500 $a Source: Dissertation Abstracts International, Volume: 67-08, Section: B, page: 4391.
502 $a Thesis (Ph.D.)--University of Waterloo (Canada), 2006.
520 $a The quest for better drug analysis may be defined in many ways, depending on the needs of the analyst. A method may be characterized by its sensitivity, specificity, accuracy, reproducibility, robustness, speed, or cost. Depending on the particular problem at hand, one characteristic may be valued above another. Given the foregoing, the overriding goal of the research presented was to develop a novel probe for in vivo monitoring of drug concentrations. The anticipated benefits were in terms of selectivity, speed of analysis due to a simplification of the sample preparation process, as well as the ability to monitor therapeutically relevant (free) drug concentrations in a dynamic living system.
520 $a These goals were achieved in the development and use of a solid phase microextraction probe employing a novel general sorbent, polypyrrole, for fast monitoring of drugs in intravenous blood. The technique was first evaluated with benzodiazepines analysis in dogs. Pharmacokinetic profiles of diazepam and it major metabolites were evaluated by both SPME and conventional sampling, with good agreement between the two methods and limits of detection <10 pg/mL. The technique was then used for the analysis of antibiotics (erythromycin and linezolid) in pigs in a simulated surgical environment.
520 $a Subsequently a highly specific probe was developed, based on immobilized anti-benzodiazepine antibodies. The probe was evaluated for benzodiazepine analysis in vitro and applied for the determination of 7-aminoflunitrazepam at pg/mL levels in spiked urine. This specific probe was capable of analyses similar to those of the general sorbent, but with higher resistance to interference from other analytes in the sample---a situation in which the general sorbent probe experiences shortcomings. In the final stage of this work a prototype in vivo probe suitable for antibody immobilization was developed based on a polyimide substrate. Its performance was found to be comparable to the in vitro probes.
520 $a The work was seen as a proof of concept as such analyses had not been previously reported. An evaluation of the results elucidates the strengths and weaknesses of the techniques developed, which will assist in refining the techniques for the most optimal applications as well as point the way for further improvements in method performance.
590 $a School code: 1141.
650 4 $a Health Sciences, Toxicology. $3 1017752
650 4 $a Chemistry, General. $3 1021807
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0383
690 $a 0485
690 $a 0486
710 2 0 $a University of Waterloo (Canada). $3 1017669
773 0 $t Dissertation Abstracts International $g 67-08B.
790 $a 1141
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NR17462