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Utilization of arrays as a monitorin...

Roling, Jonathan Adam.

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Utilization of arrays as a monitoring tool for chromium exposure at a chromium contaminated Superfund site.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Utilization of arrays as a monitoring tool for chromium exposure at a chromium contaminated Superfund site./
作者:	Roling, Jonathan Adam.
面頁冊數:	164 p.
附註:	Source: Dissertation Abstracts International, Volume: 67-04, Section: B, page: 1836.
Contained By:	Dissertation Abstracts International67-04B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3214006
ISBN:	9780542635588

Utilization of arrays as a monitoring tool for chromium exposure at a chromium contaminated Superfund site.
Roling, Jonathan Adam.

Utilization of arrays as a monitoring tool for chromium exposure at a chromium contaminated Superfund site. - 164 p.

Source: Dissertation Abstracts International, Volume: 67-04, Section: B, page: 1836.

Thesis (Ph.D.)--The University of Texas at El Paso, 2006.

The effects of chromium were analyzed on RNA expression as a biomarker in the sentinel species mummichog, Fundulus heteroclitus. Weights were measured to determine the no observable effect concentration (NOEC) and the lowest observable effect concentration (LOEC) in Fundulus at 1.5 (0.0288 mM) and 3 mg/L (0.0577 mM) for Cr(VI) and 32 (0.615 mM) and 64 mg/L (1.23 mM) for Cr(III), respectively. Mummichogs exposed to chromium demonstrated an exposure-dependent increase in body burden. In subsequent experiments, adults were exposed to Cr(VI) for 7-days at 0, 1.5 (NOEC), or 3 mg/L (LOEC), or Cr(III) at 0 or 32 mg/L (NOEC). A set of arrays were generated using sequences isolated by subtractive hybridization. The fingerprint of expression measured on the arrays in response to Cr(III) is significantly different than the fingerprint of expression caused by Cr(VI). The use of arrays as a biomonitoring tool was then assessed at a chromium-contaminated Superfund site. Chromium concentration in the sediment of Shipyard Creek significantly decreased from 1995 to 2005 due to remediation. The chromium body burden has also decreased from 2.52 mug/g in 2000, to 0.234 mug/g during remediation (2003). The number of genes differentially expressed and the magnitude of response on the array associate to the reduced body burden. Chromium body burden in concordance with gene expression increased in 2005 due to hydraulic dredging. In summary, our data supports the hypothesis that arrays can be used as multidimensional biomarkers to monitor site mitigation as the gene expression profile is associated with chromium bioavailability and body burden.

ISBN: 9780542635588Subjects--Topical Terms:

1017719
Biology, Molecular.

Utilization of arrays as a monitoring tool for chromium exposure at a chromium contaminated Superfund site.
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520 $a The effects of chromium were analyzed on RNA expression as a biomarker in the sentinel species mummichog, Fundulus heteroclitus. Weights were measured to determine the no observable effect concentration (NOEC) and the lowest observable effect concentration (LOEC) in Fundulus at 1.5 (0.0288 mM) and 3 mg/L (0.0577 mM) for Cr(VI) and 32 (0.615 mM) and 64 mg/L (1.23 mM) for Cr(III), respectively. Mummichogs exposed to chromium demonstrated an exposure-dependent increase in body burden. In subsequent experiments, adults were exposed to Cr(VI) for 7-days at 0, 1.5 (NOEC), or 3 mg/L (LOEC), or Cr(III) at 0 or 32 mg/L (NOEC). A set of arrays were generated using sequences isolated by subtractive hybridization. The fingerprint of expression measured on the arrays in response to Cr(III) is significantly different than the fingerprint of expression caused by Cr(VI). The use of arrays as a biomonitoring tool was then assessed at a chromium-contaminated Superfund site. Chromium concentration in the sediment of Shipyard Creek significantly decreased from 1995 to 2005 due to remediation. The chromium body burden has also decreased from 2.52 mug/g in 2000, to 0.234 mug/g during remediation (2003). The number of genes differentially expressed and the magnitude of response on the array associate to the reduced body burden. Chromium body burden in concordance with gene expression increased in 2005 due to hydraulic dredging. In summary, our data supports the hypothesis that arrays can be used as multidimensional biomarkers to monitor site mitigation as the gene expression profile is associated with chromium bioavailability and body burden.
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