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Analysis of dehalogenation by denitr...

Pavlik, Elizabeth Jane.

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Analysis of dehalogenation by denitrifying bacteria.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Analysis of dehalogenation by denitrifying bacteria./
作者:	Pavlik, Elizabeth Jane.
面頁冊數:	120 p.
附註:	Source: Dissertation Abstracts International, Volume: 67-01, Section: B, page: 0084.
Contained By:	Dissertation Abstracts International67-01B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3203356
ISBN:	9780542519444

Analysis of dehalogenation by denitrifying bacteria.
Pavlik, Elizabeth Jane.

Analysis of dehalogenation by denitrifying bacteria. - 120 p.

Source: Dissertation Abstracts International, Volume: 67-01, Section: B, page: 0084.

Thesis (Ph.D.)--Rutgers The State University of New Jersey - New Brunswick and University of Medicine and Dentistry of New Jersey, 2006.

Organohalide compounds are released into the environment by natural and anthropogenic sources. Significant levels of chlorinated organic compounds are produced industrially as pesticides, herbicides, fungicides and organic solvents. Degradation of organohalide compounds by denitrifying bacteria is ecologically significant due to the widespread deposition of nitrate in soils and groundwater. The combination of released halogenated compounds, and presence of nitrate, provides greater opportunity for biodegradation. This work analyzed three components of bacterial degradation of chlorinated benzoates. The first involves the characterization a novel bacterium from the Azospira genus, which has been isolated on 4-chlorobenzoate as a sole carbon and energy source, with a concomitant loss of nitrate. This bacterium is also capable of growing on 4-bromobenzoate, 4-hydroxybenzoate and benzoate, but not 3-chlorobenzoate. The second component investigates the mechanism of dechlorination of 3-chlorobenzoate by a denitrifying bacterium Thauera chlorobenzoica strain 3CB-1. Analysis of both whole cell and cell-free extract experiments suggested that dechlorination proceeded by a reductive mechanism in this bacterium. As a third component, a high-throughput screening (HTS) technique is described that correlate chloride release and pH change in whole cell systems. Optimization of the system provided conditions that allowed both a visual and spectroscopic monitoring of candidate clones for dechlorination reactions. The combination of these studies provides a multifaceted study into dehalogenation.

ISBN: 9780542519444Subjects--Topical Terms:

1017719
Biology, Molecular.

Analysis of dehalogenation by denitrifying bacteria.
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