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The role of surfactant protein D in ...

Ni, Minjian.

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The role of surfactant protein D in ocular defense against Pseudomonas aeruginosa keratitis.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The role of surfactant protein D in ocular defense against Pseudomonas aeruginosa keratitis./
作者:	Ni, Minjian.
面頁冊數:	140 p.
附註:	Source: Dissertation Abstracts International, Volume: 68-02, Section: B, page: 0900.
Contained By:	Dissertation Abstracts International68-02B.
標題:	Health Sciences, Ophthalmology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3254004

The role of surfactant protein D in ocular defense against Pseudomonas aeruginosa keratitis.
Ni, Minjian.

The role of surfactant protein D in ocular defense against Pseudomonas aeruginosa keratitis. - 140 p.

Source: Dissertation Abstracts International, Volume: 68-02, Section: B, page: 0900.

Thesis (Ph.D.)--University of California, Berkeley, 2006.

Pseudomonas aeruginosa (P. aeruginosa) keratitis is a sight threatening disease that is mostly associated with extended contact lens wearers or immunocompromised patients. Our group has earlier published the importance of human tear fluid in protecting corneal epithelial cells against P. aeruginosa infection. The work here, therefore, aimed at identifying the responsible tear component(s) and became the first to report that surfactant protein-D (SP-D) is present in both the human tear fluid and the cornea. We further characterized SP-D expression and explored the molecular mechanism of the regulation of SP-D production in human corneal epithelial cells in response to P. aeruginosa exposure.Subjects--Topical Terms:

1019445
Health Sciences, Ophthalmology.

The role of surfactant protein D in ocular defense against Pseudomonas aeruginosa keratitis.
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035 $a AAI3254004
040 $a UMI $c UMI
100 1 $a Ni, Minjian. $3 1918013
245 1 4 $a The role of surfactant protein D in ocular defense against Pseudomonas aeruginosa keratitis.
300 $a 140 p.
500 $a Source: Dissertation Abstracts International, Volume: 68-02, Section: B, page: 0900.
500 $a Adviser: Suzanne M. J. Fleiszig.
502 $a Thesis (Ph.D.)--University of California, Berkeley, 2006.
520 $a Pseudomonas aeruginosa (P. aeruginosa) keratitis is a sight threatening disease that is mostly associated with extended contact lens wearers or immunocompromised patients. Our group has earlier published the importance of human tear fluid in protecting corneal epithelial cells against P. aeruginosa infection. The work here, therefore, aimed at identifying the responsible tear component(s) and became the first to report that surfactant protein-D (SP-D) is present in both the human tear fluid and the cornea. We further characterized SP-D expression and explored the molecular mechanism of the regulation of SP-D production in human corneal epithelial cells in response to P. aeruginosa exposure.
520 $a First, we sought to identify specific tear component(s) that can confer protective effect against P. aeruginosa. Our results showed that SP-D is present in human tear fluid and involved in its protection against P. aeruginosa invasion. Western blot, RT-PCR and immunofluorescence microscopy showed that cultured human and mouse corneal epithelial cells are able to express and secrete SP-D.
520 $a Next, we demonstrated that the expression and secretion of SP-D by corneal epithelial cells are subject to regulation upon exposure to whole bacteria or bacterial components. Cultured human corneal epithelial cells challenged with heat-killed P. aeruginosa, or P. aeruginosa lipopolysaccharide or flagellin protein (FliC) showed increased expression and secretion of SP-D. This upregulation is MyD88- and NF-kappaB-independent, but JNK-, ERK- and p38-dependent. Bacteria expressing mutated FliC composed flagella, which are defective in TLR5 binding site, induced an even stronger SP-D response than bacteria with normal flagella did. Taken together, P. aeruginosa-induced SP-D and pro-inflammatory cytokine IL-8 upregulation appeared to be mediated by separate signaling pathways.
520 $a In summary, the work presented here has demonstrated that SP-D plays a significant role in ocular defense against P. aeruginosa and has shed some light on the regulation of SP-D expression at the molecular level. The findings may allow development of novel preventive methods and better therapeutic treatments for P. aeruginosa keratitis.
590 $a School code: 0028.
650 4 $a Health Sciences, Ophthalmology. $3 1019445
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Health Sciences, Immunology. $3 1017716
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710 2 0 $a University of California, Berkeley. $3 687832
773 0 $t Dissertation Abstracts International $g 68-02B.
790 1 0 $a Fleiszig, Suzanne M. J., $e advisor
790 $a 0028
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3254004