東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Genetic and biochemical analyses of ...

Sinlapadech, Taksina.

FindBook

Google Book

Amazon

博客來

Genetic and biochemical analyses of glycosyltransferases involved in Arabidopsis sinapate ester metabolism.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Genetic and biochemical analyses of glycosyltransferases involved in Arabidopsis sinapate ester metabolism./
作者:	Sinlapadech, Taksina.
面頁冊數:	113 p.
附註:	Source: Dissertation Abstracts International, Volume: 67-09, Section: B, page: 4907.
Contained By:	Dissertation Abstracts International67-09B.
標題:	Biology, Plant Physiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3232239
ISBN:	9780542866265

Genetic and biochemical analyses of glycosyltransferases involved in Arabidopsis sinapate ester metabolism.
Sinlapadech, Taksina.

Genetic and biochemical analyses of glycosyltransferases involved in Arabidopsis sinapate ester metabolism. - 113 p.

Source: Dissertation Abstracts International, Volume: 67-09, Section: B, page: 4907.

Thesis (Ph.D.)--Purdue University, 2006.

Sinapoylmalate is a major phenylpropanoid accumulated in Arabidopsis thaliana. Its presence causes leaves to fluoresce blue under UV light, and mutations that lead to lower levels of sinapoylmalate decrease UV-induced leaf fluorescence. The Arabidopsis bright trichomes 1 ( brt1) mutant was first identified in a screen for mutants that exhibit a reduced epidermal fluorescence phenotype; however, subsequent examination of the mutant revealed that its trichomes are hyperfluorescent. The results from genetic mapping and complementation analyses showed that BRT1 encodes UGT84A2, a glucosyltransferase previously shown to be capable of using sinapic acid as a substrate. Residual levels of sinapoylmalate and sinapic acid: UDP-glucose glucosyltransferase activity in brt1 leaves suggest that BRT1 is one member of a family of partially redundant glycosyltransferases that function in Arabidopsis sinapate ester biosynthesis. Reverse transcriptase-polymerase chain reaction analysis showed that BRT1 is expressed through all stages of plant life cycle, a result consistent with the impact of the brt1 mutation on both leaf sinapoylmalate levels and seed sinapoylcholine content. Identification of other glycosyltransferases that may be redundant with BRT1 has been initiated by analyses of EMS-mutagenized plants in the brt1-1 background and knockouts of UGT84A3 and UGT88A1. UV screening of EMS-mutagenized M2 plants has identified enhancers and suppressors of the brt1 mutation. A method for isolation of the compound(s) responsible for the hyperfluorescent trichome phenotype is reported.

ISBN: 9780542866265Subjects--Topical Terms:

1017865
Biology, Plant Physiology.

Genetic and biochemical analyses of glycosyltransferases involved in Arabidopsis sinapate ester metabolism.
LDR:02484nmm 2200265 4500 001 1828978
005 20071023113109.5
008 130610s2006 eng d
020 $a 9780542866265
035 $a (UMI)AAI3232239
035 $a AAI3232239
040 $a UMI $c UMI
100 1 $a Sinlapadech, Taksina. $3 1917852
245 1 0 $a Genetic and biochemical analyses of glycosyltransferases involved in Arabidopsis sinapate ester metabolism.
300 $a 113 p.
500 $a Source: Dissertation Abstracts International, Volume: 67-09, Section: B, page: 4907.
500 $a Adviser: Clint Chapple.
502 $a Thesis (Ph.D.)--Purdue University, 2006.
520 $a Sinapoylmalate is a major phenylpropanoid accumulated in Arabidopsis thaliana. Its presence causes leaves to fluoresce blue under UV light, and mutations that lead to lower levels of sinapoylmalate decrease UV-induced leaf fluorescence. The Arabidopsis bright trichomes 1 ( brt1) mutant was first identified in a screen for mutants that exhibit a reduced epidermal fluorescence phenotype; however, subsequent examination of the mutant revealed that its trichomes are hyperfluorescent. The results from genetic mapping and complementation analyses showed that BRT1 encodes UGT84A2, a glucosyltransferase previously shown to be capable of using sinapic acid as a substrate. Residual levels of sinapoylmalate and sinapic acid: UDP-glucose glucosyltransferase activity in brt1 leaves suggest that BRT1 is one member of a family of partially redundant glycosyltransferases that function in Arabidopsis sinapate ester biosynthesis. Reverse transcriptase-polymerase chain reaction analysis showed that BRT1 is expressed through all stages of plant life cycle, a result consistent with the impact of the brt1 mutation on both leaf sinapoylmalate levels and seed sinapoylcholine content. Identification of other glycosyltransferases that may be redundant with BRT1 has been initiated by analyses of EMS-mutagenized plants in the brt1-1 background and knockouts of UGT84A3 and UGT88A1. UV screening of EMS-mutagenized M2 plants has identified enhancers and suppressors of the brt1 mutation. A method for isolation of the compound(s) responsible for the hyperfluorescent trichome phenotype is reported.
590 $a School code: 0183.
650 4 $a Biology, Plant Physiology. $3 1017865
690 $a 0817
710 2 0 $a Purdue University. $3 1017663
773 0 $t Dissertation Abstracts International $g 67-09B.
790 1 0 $a Chapple, Clint, $e advisor
790 $a 0183
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3232239