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Development, QCM-D analysis and appl...

Cho, Nam-Joon.

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Development, QCM-D analysis and applications of a membrane on a chip.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Development, QCM-D analysis and applications of a membrane on a chip./
作者:	Cho, Nam-Joon.
面頁冊數:	292 p.
附註:	Source: Dissertation Abstracts International, Volume: 67-11, Section: B, page: 6245.
Contained By:	Dissertation Abstracts International67-11B.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3242531
ISBN:	9780542983085

Development, QCM-D analysis and applications of a membrane on a chip.
Cho, Nam-Joon.

Development, QCM-D analysis and applications of a membrane on a chip. - 292 p.

Source: Dissertation Abstracts International, Volume: 67-11, Section: B, page: 6245.

Thesis (Ph.D.)--Stanford University, 2006.

The goal of my research is to understand the molecular recognition between biological systems and engineering solid-support interfaces based on the lipid bilayer system. Lipid bilayers, supramolecular assemblies composing most of the biological cell membrane, segregate the intra- and extra-cellular areas of an organic cell. Proteins embedded and incorporated into the cell membrane act like specific gatekeepers for controlling the passage of nutrients, waste products, and chemical signals.{09}Understanding the characteristics of this supramolecular assembly and the molecular mechanisms controlling its formation allows its use as a biological membrane sensor in which the high specificity of inserted proteins can be exploited for molecular recognition purposes.

ISBN: 9780542983085Subjects--Topical Terms:

586156
Chemistry, Analytical.

Development, QCM-D analysis and applications of a membrane on a chip.
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500 $a Adviser: Curtis W. Frank.
502 $a Thesis (Ph.D.)--Stanford University, 2006.
520 $a The goal of my research is to understand the molecular recognition between biological systems and engineering solid-support interfaces based on the lipid bilayer system. Lipid bilayers, supramolecular assemblies composing most of the biological cell membrane, segregate the intra- and extra-cellular areas of an organic cell. Proteins embedded and incorporated into the cell membrane act like specific gatekeepers for controlling the passage of nutrients, waste products, and chemical signals.{09}Understanding the characteristics of this supramolecular assembly and the molecular mechanisms controlling its formation allows its use as a biological membrane sensor in which the high specificity of inserted proteins can be exploited for molecular recognition purposes.
520 $a To model this process, I used two different membrane systems. One is the single component phospholipid artificial membrane that provides a simple biomimetic platform. However, due to simplicity, an artificial membrane system can not account for the complexity of a physiological membrane. Therefore, I also developed a cell-derived membrane. In order to create a "membrane-on-a-chip" platform using these two membrane systems, we utilize the Quartz Crystal Microbalance-Dissipation (QCM-D) technique. QCM-D detects the kinetics of the membrane fusion and formation on solid substrates.
520 $a We collaborated with Professor Glenn's group in order to study Hepatitis C Virus (HCV). HCV is a major cause of liver disease that affects people worldwide and is the leading cause of hepatocellular carcinoma in the United States. Unfortunately, current therapies are inadequate for most of these patients, and it is hoped that a better understanding of HCV molecular virology may lead to the development of improved antiviral strategies. By developing the "membrane-on-a-chip", I am able to monitor the binding dynamics of viral protein interaction with the membranes.
520 $a In the course of researching the viral protein, amphipathic alpha-helical peptide (AH), I identified the specific action of AH peptide due to its amphipathic alpha-helical structure. Whereas previous researchers have been limited in their selection of surface materials for lipid biomembranes, the use of peptides as destabilizing agents will allow one the freedom to choose a broader variety of solid substrates to support planar bilayers. For example, the formation of model lipid bilayers supported on TiO2 substrates can be utilized in many membrane-associated biological, physiological, or electrochemical applications with the advantages provided by both supporting solid surfaces.
520 $a There is much potential in using QCM-D technique for studying protein-membrane interactions as well as a broad range of problems involving membrane proteins or lipids. The main advantage of the potential of "membrane-on-a-chip" using QCM-D that is simple and robust.
590 $a School code: 0212.
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650 4 $a Biophysics, General. $3 1019105
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