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Stable isotope dynamics of denitrifi...

Barford, Carol Chapman.

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Stable isotope dynamics of denitrification.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Stable isotope dynamics of denitrification./
作者:	Barford, Carol Chapman.
面頁冊數:	175 p.
附註:	Source: Dissertation Abstracts International, Volume: 58-09, Section: B, page: 5038.
Contained By:	Dissertation Abstracts International58-09B.
標題:	Engineering, Environmental. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9810635
ISBN:	9780591612998

Stable isotope dynamics of denitrification.
Barford, Carol Chapman.

Stable isotope dynamics of denitrification. - 175 p.

Source: Dissertation Abstracts International, Volume: 58-09, Section: B, page: 5038.

Thesis (Ph.D.)--Harvard University, 1997.

The stable isotope dynamics of denitrification were investigated via pure culture experiments and simulation modeling. To enable experiments, a chemostat-based reactor was designed and built. Gas collection and cryogenic distillation protocols were also developed. Dissimilatory (i.e. denitrification) and assimilatory nitrogen (N) budgets were kept separate by controlling culture pH. The final system allowed total N recovery between 85 and 115%.

ISBN: 9780591612998Subjects--Topical Terms:

783782
Engineering, Environmental.

Stable isotope dynamics of denitrification.
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100 1 $a Barford, Carol Chapman. $3 1914147
245 1 0 $a Stable isotope dynamics of denitrification.
300 $a 175 p.
500 $a Source: Dissertation Abstracts International, Volume: 58-09, Section: B, page: 5038.
500 $a Supervisor: Ralph Mitchell.
502 $a Thesis (Ph.D.)--Harvard University, 1997.
520 $a The stable isotope dynamics of denitrification were investigated via pure culture experiments and simulation modeling. To enable experiments, a chemostat-based reactor was designed and built. Gas collection and cryogenic distillation protocols were also developed. Dissimilatory (i.e. denitrification) and assimilatory nitrogen (N) budgets were kept separate by controlling culture pH. The final system allowed total N recovery between 85 and 115%.
520 $a Pure cultures of Paracoccus denitrificans denitrified with an overall isotope effect $( \varepsilon\sb{\rm overall}) $ of 25.8 $\ pm $ 1.4 $\ perthous $ for N isotopes. This value was found by both inter-treatment and intra-treatment based calculations. Fractionation models showed that $\ varepsilon\sb{\rm overall} $ is equivalent to the isotope effect for reduction of NO $\ sb3\sp{-} $ to NO $\ sb2\sp{-}.\ \varepsilon\sb{\rm overall} $ was constant for dissolved oxygen concentrations between 0 and 1.2 $\ mu M, $ which helps validate the use of $\ varepsilon\sb{\rm overall} $ for studying denitrification along oxygen gradients.
520 $a An $\ varepsilon $ of 10.2 $\ pm $ 3.2 $\ perthous $ was found for N $\ sb2 $\ varepsilon $ currently exist in the literature. It enables calculation of $\ delta\sp{15} $\ sb2 $\ varepsilon\sb{\rm overall} $ alone. This $\ varepsilon $ was also constant with respect to dissolved oxygen concentration.
520 $a Oxygen isotope effects were calculated from N $\ sb2 $\ varepsilon\sb{\rm overall} $ for oxygen is a linear combination of the fractionation within the pathway $( \varepsilon =14 $ to 19 $\ perthous $) , and fractionation associated with oxygen atoms leaving the pathway (combined $\ varepsilon =122\perthous). $ The resultant $\ varepsilon\sb{\rm overall} $ is much larger than other estimates in the literature.
520 $a The potential use of stable isotope information to control N removal or N $\ sb2 $\ rm\delta\sp{15}N\sb2O $ produced by linked nitrification-denitrification in specific treatment plant configurations. Results suggested that maximum N removal and net N $\ sb2 $\ rm\delta\sp{15}N\sb2O. $ The application of these results, as well as methods developed in this thesis, are discussed in the context of engineering design and operation.
590 $a School code: 0084.
650 4 $a Engineering, Environmental. $3 783782
650 4 $a Biogeochemistry. $3 545717
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0775
690 $a 0425
690 $a 0410
710 2 0 $a Harvard University. $3 528741
773 0 $t Dissertation Abstracts International $g 58-09B.
790 1 0 $a Mitchell, Ralph, $e advisor
790 $a 0084
791 $a Ph.D.
792 $a 1997
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9810635