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The influence of cholesterol loading...

Anderson, Crystal R.

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The influence of cholesterol loading and subsequent unloading in preservation of stallion spermatozoa.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The influence of cholesterol loading and subsequent unloading in preservation of stallion spermatozoa./
Author:	Anderson, Crystal R.
Description:	58 p.
Notes:	Source: Masters Abstracts International, Volume: 43-05, page: 1643.
Contained By:	Masters Abstracts International43-05.
Subject:	Agriculture, Animal Culture and Nutrition. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1430980
ISBN:	9780542025679

The influence of cholesterol loading and subsequent unloading in preservation of stallion spermatozoa.
Anderson, Crystal R.

The influence of cholesterol loading and subsequent unloading in preservation of stallion spermatozoa. - 58 p.

Source: Masters Abstracts International, Volume: 43-05, page: 1643.

Thesis (M.S.)--The University of Arizona, 2005.

The purpose of this study was to determine the influence of loading cholesterol into the stallion spermatozoa membrane prior to cold storage or cryopreservation, followed by the subsequent unloading of cholesterol from the membrane after preservation. Cholesterol loaded cyclodextrin (CLC) was added to spermatozoa samples before preservation to incorporate cholesterol into the membrane. Methyl beta cyclodextrin (MbetaCD) was added following preservation to remove excess cholesterol that may inhibit the potential for fertilization. Experiment I consisted of dose response trials to determine the optimal amount of CLC and MbetaCD to include based on percentages of progressively motile spermatozoa (PMS) following cold storage or cryopreservation. In experiment II, the influences of CLC and MbetaCD on PMS, the percentages of live intact (LI) and live non-intact (LNI) spermatozoa were determined following 24 and 48 h of cold storage of spermatozoa cooled in the presence or absence of seminal plasma. Experiment III consisted of cholesterol loading prior to cryopreservation and subsequent removal of cholesterol post-thaw. (Abstract shortened by UMI.)

ISBN: 9780542025679Subjects--Topical Terms:

1017857
Agriculture, Animal Culture and Nutrition.

The influence of cholesterol loading and subsequent unloading in preservation of stallion spermatozoa.
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100 1 $a Anderson, Crystal R. $3 1914094
245 1 4 $a The influence of cholesterol loading and subsequent unloading in preservation of stallion spermatozoa.
300 $a 58 p.
500 $a Source: Masters Abstracts International, Volume: 43-05, page: 1643.
500 $a Adviser: Mark J. Arns.
502 $a Thesis (M.S.)--The University of Arizona, 2005.
520 $a The purpose of this study was to determine the influence of loading cholesterol into the stallion spermatozoa membrane prior to cold storage or cryopreservation, followed by the subsequent unloading of cholesterol from the membrane after preservation. Cholesterol loaded cyclodextrin (CLC) was added to spermatozoa samples before preservation to incorporate cholesterol into the membrane. Methyl beta cyclodextrin (MbetaCD) was added following preservation to remove excess cholesterol that may inhibit the potential for fertilization. Experiment I consisted of dose response trials to determine the optimal amount of CLC and MbetaCD to include based on percentages of progressively motile spermatozoa (PMS) following cold storage or cryopreservation. In experiment II, the influences of CLC and MbetaCD on PMS, the percentages of live intact (LI) and live non-intact (LNI) spermatozoa were determined following 24 and 48 h of cold storage of spermatozoa cooled in the presence or absence of seminal plasma. Experiment III consisted of cholesterol loading prior to cryopreservation and subsequent removal of cholesterol post-thaw. (Abstract shortened by UMI.)
590 $a School code: 0009.
650 4 $a Agriculture, Animal Culture and Nutrition. $3 1017857
650 4 $a Biology, Animal Physiology. $3 1017835
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710 2 0 $a The University of Arizona. $3 1017508
773 0 $t Masters Abstracts International $g 43-05.
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791 $a M.S.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1430980