語系:
繁體中文
English
說明(常見問題)
回圖書館首頁
手機版館藏查詢
登入
回首頁
切換:
標籤
|
MARC模式
|
ISBD
Characterization of the human Ig gue...
~
Duteau, Anae.
FindBook
Google Book
Amazon
博客來
Characterization of the human Ig guest locus in HAC transgenic cattle.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Characterization of the human Ig guest locus in HAC transgenic cattle./
作者:
Duteau, Anae.
面頁冊數:
146 p.
附註:
Source: Dissertation Abstracts International, Volume: 66-06, Section: B, page: 3041.
Contained By:
Dissertation Abstracts International66-06B.
標題:
Health Sciences, Immunology. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3179871
ISBN:
9780542197574
Characterization of the human Ig guest locus in HAC transgenic cattle.
Duteau, Anae.
Characterization of the human Ig guest locus in HAC transgenic cattle.
- 146 p.
Source: Dissertation Abstracts International, Volume: 66-06, Section: B, page: 3041.
Thesis (Ph.D.)--University of Massachusetts Amherst, 2005.
Human, antigen-specific polyclonal antibodies are in high demand for therapeutic and research applications. However, the supply of these antibodies currently comes from only human donors and cannot satisfy the demand. The application of pathogen-specific human polyclonal antibodies is limited because there are severe restrictions on the kinds of antigens and the immunization protocols that can be used in humans. In the attempt to resolve the issue of the supply of antigen-specific, human polyclonal antibodies, transgenic for human Ig (hIg) animals were created. The most sufficient and promising model was the human artificial chromosome (HAC) transgenic animal. This model was successful due to the most efficient transferring vector CS20, comprising the entire unrearranged human heavy (1.5MB) and lambda locus (1 MB). In order to produce a large quantity of polyclonal hIg, cloned HAC transgenic cattle were created. Highly human-specific and bovine non-cross reactive, polyclonal bovine anti-human antibody and sensitive solid phase ELISA were created to determine, quantify, and characterize hIg in the sera of HAC transgenic cattle. Using the assay, that have been developed in this study; it was found that the majority of the HAC cattle produce hIg. It was also found that both heavy and light chains of hIg are produced by HAC bovines. The heavy chain of hIg undergoes class switching to the IgG and its half-life is 30 days, which is longer than hIgG in humans (21 days) or bovine IgG in bovines (19 days). Highly human-specific and bovine non-cross reactive monoclonal antibodies for the characterization of hIg produced by HAC cattle were created to recognize hIg heavy chain classes/subclasses and light chains. Analysis of the human V lambda genes sequences derived from HAC transgenic cattle demonstrated that human genes undergo extensive rearrangement and somatic hypermutation following normal Ig patterns. This study has demonstrated that hIg produced by HAC cattle diversifies according to normal Ig patterns and undergoes class switching to IgG. The half-life of hIgG is sufficiently long for protection from pathogens of the homozygous Ig deficient HAC cattle, and for harvesting of human IgG. HAC transgenic cattle can be potential donors of human polyclonal Ig.
ISBN: 9780542197574Subjects--Topical Terms:
1017716
Health Sciences, Immunology.
Characterization of the human Ig guest locus in HAC transgenic cattle.
LDR
:03215nmm 2200289 4500
001
1825059
005
20061205094422.5
008
130610s2005 eng d
020
$a
9780542197574
035
$a
(UnM)AAI3179871
035
$a
AAI3179871
040
$a
UnM
$c
UnM
100
1
$a
Duteau, Anae.
$3
1914083
245
1 0
$a
Characterization of the human Ig guest locus in HAC transgenic cattle.
300
$a
146 p.
500
$a
Source: Dissertation Abstracts International, Volume: 66-06, Section: B, page: 3041.
500
$a
Director: Richard A. Goldsby.
502
$a
Thesis (Ph.D.)--University of Massachusetts Amherst, 2005.
520
$a
Human, antigen-specific polyclonal antibodies are in high demand for therapeutic and research applications. However, the supply of these antibodies currently comes from only human donors and cannot satisfy the demand. The application of pathogen-specific human polyclonal antibodies is limited because there are severe restrictions on the kinds of antigens and the immunization protocols that can be used in humans. In the attempt to resolve the issue of the supply of antigen-specific, human polyclonal antibodies, transgenic for human Ig (hIg) animals were created. The most sufficient and promising model was the human artificial chromosome (HAC) transgenic animal. This model was successful due to the most efficient transferring vector CS20, comprising the entire unrearranged human heavy (1.5MB) and lambda locus (1 MB). In order to produce a large quantity of polyclonal hIg, cloned HAC transgenic cattle were created. Highly human-specific and bovine non-cross reactive, polyclonal bovine anti-human antibody and sensitive solid phase ELISA were created to determine, quantify, and characterize hIg in the sera of HAC transgenic cattle. Using the assay, that have been developed in this study; it was found that the majority of the HAC cattle produce hIg. It was also found that both heavy and light chains of hIg are produced by HAC bovines. The heavy chain of hIg undergoes class switching to the IgG and its half-life is 30 days, which is longer than hIgG in humans (21 days) or bovine IgG in bovines (19 days). Highly human-specific and bovine non-cross reactive monoclonal antibodies for the characterization of hIg produced by HAC cattle were created to recognize hIg heavy chain classes/subclasses and light chains. Analysis of the human V lambda genes sequences derived from HAC transgenic cattle demonstrated that human genes undergo extensive rearrangement and somatic hypermutation following normal Ig patterns. This study has demonstrated that hIg produced by HAC cattle diversifies according to normal Ig patterns and undergoes class switching to IgG. The half-life of hIgG is sufficiently long for protection from pathogens of the homozygous Ig deficient HAC cattle, and for harvesting of human IgG. HAC transgenic cattle can be potential donors of human polyclonal Ig.
590
$a
School code: 0118.
650
4
$a
Health Sciences, Immunology.
$3
1017716
650
4
$a
Agriculture, Animal Culture and Nutrition.
$3
1017857
650
4
$a
Biology, Veterinary Science.
$3
1021733
690
$a
0982
690
$a
0475
690
$a
0778
710
2 0
$a
University of Massachusetts Amherst.
$3
1019433
773
0
$t
Dissertation Abstracts International
$g
66-06B.
790
1 0
$a
Goldsby, Richard A.,
$e
advisor
790
$a
0118
791
$a
Ph.D.
792
$a
2005
856
4 0
$u
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3179871
筆 0 讀者評論
館藏地:
全部
電子資源
出版年:
卷號:
館藏
1 筆 • 頁數 1 •
1
條碼號
典藏地名稱
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
W9215922
電子資源
11.線上閱覽_V
電子書
EB
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
評論
新增評論
分享你的心得
Export
取書館
處理中
...
變更密碼
登入