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Bioconversion of corn fibre to ethanol.

Bura, Renata.

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Bioconversion of corn fibre to ethanol.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Bioconversion of corn fibre to ethanol./
作者:	Bura, Renata.
面頁冊數:	204 p.
附註:	Source: Dissertation Abstracts International, Volume: 66-01, Section: B, page: 0027.
Contained By:	Dissertation Abstracts International66-01B.
標題:	Agriculture, Wood Technology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ99448
ISBN:	9780612994485

Bioconversion of corn fibre to ethanol.
Bura, Renata.

Bioconversion of corn fibre to ethanol. - 204 p.

Source: Dissertation Abstracts International, Volume: 66-01, Section: B, page: 0027.

Thesis (Ph.D.)--The University of British Columbia (Canada), 2005.

Corn fibre, due to its chemical and structural properties, was evaluated as a potential technically viable feedstock that might be used to demonstrate the effectiveness of a biomass to ethanol bioconversion process. One of the major goals of the thesis was to see if we could define the optimum pretreatment conditions which would result in maximal sugar recovery and ethanol production, with a minimum number of subprocess steps.

ISBN: 9780612994485Subjects--Topical Terms:

1031154
Agriculture, Wood Technology.

Bioconversion of corn fibre to ethanol.
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500 $a Source: Dissertation Abstracts International, Volume: 66-01, Section: B, page: 0027.
500 $a Adviser: J. N. Saddler.
502 $a Thesis (Ph.D.)--The University of British Columbia (Canada), 2005.
520 $a Corn fibre, due to its chemical and structural properties, was evaluated as a potential technically viable feedstock that might be used to demonstrate the effectiveness of a biomass to ethanol bioconversion process. One of the major goals of the thesis was to see if we could define the optimum pretreatment conditions which would result in maximal sugar recovery and ethanol production, with a minimum number of subprocess steps.
520 $a Corn fibre was pretreated using SO2-catalysed steam explosion at thirteen different severity conditions, chosen based on response surface modeling, with the goal of finding optimal pretreatment conditions. These conditions were defined to allow us to recover most of the hexose hemicellulose and cellulose derived sugars in an hydrolysable and fermentable form. The chosen severity conditions had a pronounced effect on the total amount of sugars recovered from corn fibre. Due to the chemical and biological nature of corn fibre (low lignin and high carbohydrate content), we were able to establish optimum, mild steam pretreatment conditions---190°C for 5 minutes after exposure to 3% SO2---which yielded 93% of the total sugars in a hemicellulose-rich water soluble fraction, and a readily hydrolysable cellulose fraction by enzymes. The optimum pretreatment conditions also resulted in the production of a limited amount of sugar decomposition products, which allowed us to effectively ferment hexose sugars to ethanol using Saccharomyces cerevisiae.
520 $a Efforts to increase the low sugar concentrations in the water soluble fraction were evaluated in an attempt to increase the final ethanol concentrations recovered following fermentation. Increasing the sugar concentration by using a high consistency (12% w/v) cellulose-rich water insoluble fraction in acetate buffer resulted in the generation of a glucose stream of 55 g L-1 . However, mixing this higher concentration water insoluble, cellulose stream was problematic, as evident by incomplete hydrolysis. As an alternative strategy, the hemicellulose-rich water soluble fraction was added to the cellulose-rich stream, effectively increasing the sugar concentration while reducing the number of subprocess steps to pretreatment, hydrolysis and fermentation. Although combining the hemicellulose and cellulose streams increased the overall sugar concentration, cellulose hydrolysis was inhibited due to end-product inhibition. Increased hydrolysis time, and enzyme loadings were able to further increase cellulose hydrolysis yields.
520 $a In an effort to increase overall cellulose conversion, minimize end-product inhibition, and simplify the overall process, the simultaneous saccharification and fermentation of the entire slurry after steam explosion was investigated. (Abstract shortened by UMI.)
590 $a School code: 2500.
650 4 $a Agriculture, Wood Technology. $3 1031154
650 4 $a Engineering, Agricultural. $3 1019504
690 $a 0746
690 $a 0539
710 2 0 $a The University of British Columbia (Canada). $3 626643
773 0 $t Dissertation Abstracts International $g 66-01B.
790 1 0 $a Saddler, J. N., $e advisor
790 $a 2500
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ99448