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Detection of bovine herpesvirus 1 (B...

Zhou, Jianwei.

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Detection of bovine herpesvirus 1 (BHV 1) in bovine semen by combined polymerase chain reaction and in vitro protein synthesis.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Detection of bovine herpesvirus 1 (BHV 1) in bovine semen by combined polymerase chain reaction and in vitro protein synthesis./
作者:	Zhou, Jianwei.
面頁冊數:	91 p.
附註:	Source: Masters Abstracts International, Volume: 37-03, page: 0870.
Contained By:	Masters Abstracts International37-03.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ35465
ISBN:	9780612354654

Detection of bovine herpesvirus 1 (BHV 1) in bovine semen by combined polymerase chain reaction and in vitro protein synthesis.
Zhou, Jianwei.

Detection of bovine herpesvirus 1 (BHV 1) in bovine semen by combined polymerase chain reaction and in vitro protein synthesis. - 91 p.

Source: Masters Abstracts International, Volume: 37-03, page: 0870.

Thesis (M.Sc.)--University of Prince Edward Island (Canada), 1998.

Bovine herpesvirus 1 (BHV 1) is a member of the family Herpesviridae, subfamily Alphaherpesvirinae. BHV 1 is an important cause of infectious bovine rhinotracheitis, infectious pustular vulvovaginitis and infectious pustular balanoposthitis. BHV 1 is frequently found in bovine semen and transmitted through artificial insemination. Semen is most likely contaminated during ejaculation by virus that is shed from the infected mucosa. Artificial insemination with BHV 1 contaminated semen can result in markedly reduced conception rates, shortened oestrous cycles, and endometritis. The linear-double stranded DNA genome of BHV 1 consists of approximately 140 kilobase pairs (kbp). Glycoprotein D (gD), a glycoprotein of 71kD, has been identified as a suitable candidate for the production of subunit vaccines, and as a major molecule on the surface of the virion and virus-infected cells. It induces a strong and consistent humoral and cellular immune response to BHV 1. The main objective of this study was to develop an improved method for detecting BHV 1 in bovine semen samples. The polymerase chain reaction (PCR) products amplified from the gD gene region of BHV 1 were transcribed and translated into gD peptides in an attempt to improve the sensitivity of direct virus detection in clinical samples. (Abstract shortened by UMI.)

ISBN: 9780612354654Subjects--Topical Terms:

1017734
Biology, Microbiology.

Detection of bovine herpesvirus 1 (BHV 1) in bovine semen by combined polymerase chain reaction and in vitro protein synthesis.
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