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Coat protein gene identification, ge...

Ling, Kai-Shu.

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Coat protein gene identification, genome organization, and PCR detection of grapevine leafroll associated closterovirus-3 and study towards transgenic grapevines.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Coat protein gene identification, genome organization, and PCR detection of grapevine leafroll associated closterovirus-3 and study towards transgenic grapevines./
作者:	Ling, Kai-Shu.
面頁冊數:	232 p.
附註:	Source: Dissertation Abstracts International, Volume: 57-03, Section: B, page: 1539.
Contained By:	Dissertation Abstracts International57-03B.
標題:	Agriculture, Plant Pathology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9624860

Coat protein gene identification, genome organization, and PCR detection of grapevine leafroll associated closterovirus-3 and study towards transgenic grapevines.
Ling, Kai-Shu.

Coat protein gene identification, genome organization, and PCR detection of grapevine leafroll associated closterovirus-3 and study towards transgenic grapevines. - 232 p.

Source: Dissertation Abstracts International, Volume: 57-03, Section: B, page: 1539.

Thesis (Ph.D.)--Cornell University, 1995.

Leafroll is one of the most important virus diseases of grape (Vitis sp.). Although closteroviruses are closely associated with leafroll, the etiology of the disease is yet to be determined. These closteroviruses are serologically distinct and designated as grapevine leafroll associated viruses (GLRaV) Subjects--Topical Terms:

1028950
Agriculture, Plant Pathology.

Coat protein gene identification, genome organization, and PCR detection of grapevine leafroll associated closterovirus-3 and study towards transgenic grapevines.
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035 $a (UnM)AAI9624860
035 $a AAI9624860
040 $a UnM $c UnM
100 1 $a Ling, Kai-Shu. $3 1913379
245 1 0 $a Coat protein gene identification, genome organization, and PCR detection of grapevine leafroll associated closterovirus-3 and study towards transgenic grapevines.
300 $a 232 p.
500 $a Source: Dissertation Abstracts International, Volume: 57-03, Section: B, page: 1539.
500 $a Adviser: Dennis Gonsalves.
502 $a Thesis (Ph.D.)--Cornell University, 1995.
520 $a Leafroll is one of the most important virus diseases of grape (Vitis sp.). Although closteroviruses are closely associated with leafroll, the etiology of the disease is yet to be determined. These closteroviruses are serologically distinct and designated as grapevine leafroll associated viruses (GLRaV) $- $1 to $- $6 .
520 $a In the present study, a cDNA library was prepared to the NY1 isolate of GLRaV-3 by cloning its specific double-stranded RNA into a protein expression vector lambda ZAPII. Based on the sequence of three immunopositive clones, a common open reading frame encoding a protein of 35K was tentatively identified as the coat protein gene of GLRaV-3. A total of 12,930 nucleotides, representing about 80% of the genome, have been sequenced. Analysis of the GLRaV-3 genome organization demonstrated a close similarity to other closteroviruses. At least nine ORFs were tentatively identified and designated as: ORF 1a ( $> $1 49K, including a helicase domain), ORF 1b (61K, RNA dependent RNA polymerase), ORF 2 (7K, unknown function), ORF 3 (5K, transmembrane protein), ORF 4 (59K, HSP70 homologue), ORF 5 (56K, HSP90 homologue), ORF 6 (35K, coat protein), ORF 7 (20K, diverged copy of coat protein), and ORF 8 ( $> $3 8K, putative ambisense gene). According to other closteroviruses, ORF 1b of GLRaV-3 may also be expressed via a +1 ribosomal frameshift mechanism which is similar to that of lettuce infectious yellows virus. Detection of GLRaV-3 by polymerase chain reaction (PCR) was developed; immuno-capture PCR or reverse transcription-PCR with proteinase K treated crude extract were useful. Nested-PCR was further developed to achieve a higher standard in the specificity for diagnosing GLRaV. In the study towards genetically engineered protection of grapevines, a 43K fragment of ORF 5 was engineered. Transgenic tobacco plants that contained the gene were obtained after Agrobacterium transformation.
590 $a School code: 0058.
650 4 $a Agriculture, Plant Pathology. $3 1028950
650 4 $a Biology, Molecular. $3 1017719
690 $a 0480
690 $a 0307
710 2 0 $a Cornell University. $3 530586
773 0 $t Dissertation Abstracts International $g 57-03B.
790 1 0 $a Gonsalves, Dennis, $e advisor
790 $a 0058
791 $a Ph.D.
792 $a 1995
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9624860