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Effects of phosphated titanium and e...

Dacy, Justin Anthony.

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Effects of phosphated titanium and enamel matrix derivatives on osteoblast behavior in vitro.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Effects of phosphated titanium and enamel matrix derivatives on osteoblast behavior in vitro./
作者:	Dacy, Justin Anthony.
面頁冊數:	66 p.
附註:	Source: Masters Abstracts International, Volume: 44-04, page: 1804.
Contained By:	Masters Abstracts International44-04.
標題:	Health Sciences, Dentistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1432164
ISBN:	9780542505058

Effects of phosphated titanium and enamel matrix derivatives on osteoblast behavior in vitro.
Dacy, Justin Anthony.

Effects of phosphated titanium and enamel matrix derivatives on osteoblast behavior in vitro. - 66 p.

Source: Masters Abstracts International, Volume: 44-04, page: 1804.

Thesis (M.S.)--The Texas A&M University System Health Science Center, 2006.

The effects of phosphated titanium and enamel matrix derivatives (EMD) were evaluated on osteoblast attachment and function. Primary rat osteoblasts were cultured on phosphated or non-phosphated titanium for 28 days; half the samples received EMD. Analysis was done using TGF-beta1 and IL-1beta ELISAs, scanning electron microscopy and light microscopy. Microscopic evaluation revealed osteoblasts increasing in number on all discs from day 2 to 28. Nodule formation occurred in all groups and when EMD was added numbers increased. Without EMD and mineralizing media, non-phosphated samples had 2-3 times more nodules; none showed evidence of mineralization. All nodules on the phosphated titanium had evidence of mineralization. ELISA analysis revealed no significant differences in IL-1beta production between any of the groups and the EMD groups produced significantly more TGF-beta1 for up to 8 days. Phosphated titanium increased TGF-beta1 and did not adversely affect early osteoblast attachment and function, but accelerated nodule mineralization.

ISBN: 9780542505058Subjects--Topical Terms:

1019378
Health Sciences, Dentistry.

Effects of phosphated titanium and enamel matrix derivatives on osteoblast behavior in vitro.
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520 $a The effects of phosphated titanium and enamel matrix derivatives (EMD) were evaluated on osteoblast attachment and function. Primary rat osteoblasts were cultured on phosphated or non-phosphated titanium for 28 days; half the samples received EMD. Analysis was done using TGF-beta1 and IL-1beta ELISAs, scanning electron microscopy and light microscopy. Microscopic evaluation revealed osteoblasts increasing in number on all discs from day 2 to 28. Nodule formation occurred in all groups and when EMD was added numbers increased. Without EMD and mineralizing media, non-phosphated samples had 2-3 times more nodules; none showed evidence of mineralization. All nodules on the phosphated titanium had evidence of mineralization. ELISA analysis revealed no significant differences in IL-1beta production between any of the groups and the EMD groups produced significantly more TGF-beta1 for up to 8 days. Phosphated titanium increased TGF-beta1 and did not adversely affect early osteoblast attachment and function, but accelerated nodule mineralization.
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