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Transformation of American elm with ...
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Newhouse, Andrew E.
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Transformation of American elm with a gene encoding a synthetic antimicrobial peptide for resistance to Dutch-elm disease.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Transformation of American elm with a gene encoding a synthetic antimicrobial peptide for resistance to Dutch-elm disease./
作者:
Newhouse, Andrew E.
面頁冊數:
97 p.
附註:
Source: Masters Abstracts International, Volume: 44-04, page: 1722.
Contained By:
Masters Abstracts International44-04.
標題:
Agriculture, Forestry and Wildlife. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1430685
ISBN:
9780542479397
Transformation of American elm with a gene encoding a synthetic antimicrobial peptide for resistance to Dutch-elm disease.
Newhouse, Andrew E.
Transformation of American elm with a gene encoding a synthetic antimicrobial peptide for resistance to Dutch-elm disease.
- 97 p.
Source: Masters Abstracts International, Volume: 44-04, page: 1722.
Thesis (M.S.)--State University of New York College of Environmental Science and Forestry, 2005.
American elms were a nearly ubiquitous part of both urban and rural life in the United States until Dutch-elm disease, caused by the introduced fungus Ophiostoma novo-ulmi, killed almost all mature trees in the mid-twentieth century. In this project, Agrobacterium was used to transform American elm with a vascular-expressed gene encoding the synthetic antimicrobial peptide ESF39A. At least three unique, single-copy transgenic lines were produced and regenerated into whole plants, which showed significantly less wilting and sapwood staining than non-transformed controls after O. novo-ulmi inoculation. Copy number was determined by Southern hybridization, expression was confirmed by RT-PCR, and peptide activity against O. novo-ulmi was tested with an in vitro 'minimum inhibitory concentration' assay. Preliminary observations indicated that mycorrhizal colonization was not significantly different between transgenic and wild-type trees. These results indicate that transgenes encoding antimicrobial peptides hold promise for enhancing pathogen resistance in American elm.
ISBN: 9780542479397Subjects--Topical Terms:
783690
Agriculture, Forestry and Wildlife.
Transformation of American elm with a gene encoding a synthetic antimicrobial peptide for resistance to Dutch-elm disease.
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American elms were a nearly ubiquitous part of both urban and rural life in the United States until Dutch-elm disease, caused by the introduced fungus Ophiostoma novo-ulmi, killed almost all mature trees in the mid-twentieth century. In this project, Agrobacterium was used to transform American elm with a vascular-expressed gene encoding the synthetic antimicrobial peptide ESF39A. At least three unique, single-copy transgenic lines were produced and regenerated into whole plants, which showed significantly less wilting and sapwood staining than non-transformed controls after O. novo-ulmi inoculation. Copy number was determined by Southern hybridization, expression was confirmed by RT-PCR, and peptide activity against O. novo-ulmi was tested with an in vitro 'minimum inhibitory concentration' assay. Preliminary observations indicated that mycorrhizal colonization was not significantly different between transgenic and wild-type trees. These results indicate that transgenes encoding antimicrobial peptides hold promise for enhancing pathogen resistance in American elm.
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