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Functional proteomics in Escherichia...

Champion, Matthew Maurice.

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Functional proteomics in Escherichia coli.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Functional proteomics in Escherichia coli./
作者:	Champion, Matthew Maurice.
面頁冊數:	211 p.
附註:	Source: Dissertation Abstracts International, Volume: 66-12, Section: B, page: 6590.
Contained By:	Dissertation Abstracts International66-12B.
標題:	Chemistry, Biochemistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3202298
ISBN:	9780542473753

Functional proteomics in Escherichia coli.
Champion, Matthew Maurice.

Functional proteomics in Escherichia coli. - 211 p.

Source: Dissertation Abstracts International, Volume: 66-12, Section: B, page: 6590.

Thesis (Ph.D.)--Texas A&M University, 2005.

Cells respond to their environment with programmed changes in gene expression. Cataloging these changes at the protein level is key towards understanding the physiology of an organism. Multi-subunit and multi-protein complexes are also important and pathogenic and physiologic processes. In order to identify expressed proteins and potential protein complexes, we utilized a combination of non-denaturing chromatography and peptide mass fingerprinting. This approach allows us to identify the components of protein mixtures, as well as information lost in traditional proteomics, such as subunit associations. Applying this methodology to cells at both mid-exponential and stationary phase growth conditions, we identified several thousand proteins from each cell-state of E. coli corresponding to hundreds of unique gene products. The copurification of proteins when fractionated at varying pHs could suggest the components of higher order complexes. This non-denaturing proteomic approach should provide physiological data unavailable by other means. The components of several known cellular complexes were also evident in this analysis. To characterize proteins associated with nucleic acid binding, we also performed proteome analysis on log and stationary phase cells grown in LB separated over heparin chromatography at neutral pH, which enriches for these proteins. The complete analysis of these identifications is discussed.

ISBN: 9780542473753Subjects--Topical Terms:

1017722
Chemistry, Biochemistry.

Functional proteomics in Escherichia coli.
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