東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Surfactant based membrane perturbati...

Apel-Paz, Meirav.

Linked to FindBook

Google Book

Amazon

博客來

Surfactant based membrane perturbation as a strategy for contraception and STD prevention.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Surfactant based membrane perturbation as a strategy for contraception and STD prevention./
Author:	Apel-Paz, Meirav.
Description:	216 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-02, Section: B, page: 1023.
Contained By:	Dissertation Abstracts International66-02B.
Subject:	Engineering, Chemical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3164959
ISBN:	9780542000782

Surfactant based membrane perturbation as a strategy for contraception and STD prevention.
Apel-Paz, Meirav.

Surfactant based membrane perturbation as a strategy for contraception and STD prevention. - 216 p.

Source: Dissertation Abstracts International, Volume: 66-02, Section: B, page: 1023.

Thesis (Ph.D.)--Princeton University, 2005.

Prominent and effective means of pathogen attack are based on membrane perturbation. Surfactants are routinely used in this capacity, especially for contraception and the prevention of sexually transmitted diseases. Ideally, an membrane active agent should selectively perturb sperm and pathogen membranes in favor of healthy epithelial cells or the lactobacilli of the vaginal microflora.

ISBN: 9780542000782Subjects--Topical Terms:

1018531
Engineering, Chemical.

Surfactant based membrane perturbation as a strategy for contraception and STD prevention.
LDR:03376nmm 2200313 4500 001 1821547
005 20061114121657.5
008 130610s2005 eng d
020 $a 9780542000782
035 $a (UnM)AAI3164959
035 $a AAI3164959
040 $a UnM $c UnM
100 1 $a Apel-Paz, Meirav. $3 1910726
245 1 0 $a Surfactant based membrane perturbation as a strategy for contraception and STD prevention.
300 $a 216 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-02, Section: B, page: 1023.
500 $a Adviser: T. Kyle Vanderlick.
502 $a Thesis (Ph.D.)--Princeton University, 2005.
520 $a Prominent and effective means of pathogen attack are based on membrane perturbation. Surfactants are routinely used in this capacity, especially for contraception and the prevention of sexually transmitted diseases. Ideally, an membrane active agent should selectively perturb sperm and pathogen membranes in favor of healthy epithelial cells or the lactobacilli of the vaginal microflora.
520 $a This thesis describes experimental work using leakage assays and isothermal titration calorimetry as applied to lipid vesicles to better understand the interactions between membranes and leading surfactant candidates for contraceptive/STD applications: nonoxynol-9 (N-9), C31G, benzalkonium chloride (BZK), and sodium dodecyl sulfate (SDS).
520 $a We find that uptake of surfactant into the membrane results in continuous leakage of vesicle contents, following first-order kinetics. Surfactants which readily translocate across the two leaflets of the lipid bilayer (N-9, C31G, BZK) were found to promote much higher rates of leakage as compared to SDS which does not "flip-flop" on experimental time scales. For surfactants which do flip-flop, the rate of leakage depends primarily on the ratio of membrane-bound surfactant to lipid. The rate constants exhibit a power-law dependence on surfactant concentration, suggesting a cooperative mechanism for surfactant-mediated transport of solutes across the membrane. When surfactant is present at concentrations sufficient to form micelles, the rates of leakage increase markedly.
520 $a Our work also capitalizes on the isolation of the plasma membrane from rabbit sperm cells and on our efforts to create a synthetic lipid mixture as a model for the sperm plasma membrane. In particular, we show that certain lipid constituents unique to sperm membranes (e.g., a highly unsaturated ether-linked phospholipid) can indeed be associated with a decrease in membrane resistance towards certain perturbants (e.g. BZK). Meanwhile, the surfactant currently used in commercial spermicides, N-9, displays no specific interactions with sperm membranes over less complicated membranes as long as the amount of cholesterol is similar. The presence of cholesterol plays a significant role in regulating membrane permeability regardless of the surfactant used, with permeability decreasing in proportion to cholesterol content. Similar effects were seen with other sterols (dihydrocholesterol and coprostanol).
590 $a School code: 0181.
650 4 $a Engineering, Chemical. $3 1018531
650 4 $a Biophysics, General. $3 1019105
690 $a 0542
690 $a 0786
710 2 0 $a Princeton University. $3 645579
773 0 $t Dissertation Abstracts International $g 66-02B.
790 1 0 $a Vanderlick, T. Kyle, $e advisor
790 $a 0181
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3164959