東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Protein-ligand and protein-protein i...

Kim, Young-Chan.

FindBook

Google Book

Amazon

博客來

Protein-ligand and protein-protein interactions involved in de novo initiation of RNA synthesis by the hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp).

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Protein-ligand and protein-protein interactions involved in de novo initiation of RNA synthesis by the hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp)./
作者:	Kim, Young-Chan.
面頁冊數:	136 p.
附註:	Source: Dissertation Abstracts International, Volume: 67-01, Section: B, page: 0249.
Contained By:	Dissertation Abstracts International67-01B.
標題:	Chemistry, Biochemistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3204540
ISBN:	9780542504754

Protein-ligand and protein-protein interactions involved in de novo initiation of RNA synthesis by the hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp).
Kim, Young-Chan.

Protein-ligand and protein-protein interactions involved in de novo initiation of RNA synthesis by the hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp). - 136 p.

Source: Dissertation Abstracts International, Volume: 67-01, Section: B, page: 0249.

Thesis (Ph.D.)--Indiana University, 2006.

Hepatitis C Virus (HCV) is a major etiological agent of non-A, non-B chronic hepatitis. An estimated 180 million people worldwide are infected with this virus. Given this huge epidemic and relative low efficacy of current treatment for hepatitis C, it is urgent to develop better antiviral therapeutics. Better understanding of the mechanism of all aspect of RNA-dependent RNA synthesis will contributes for this purpose. A feature unique to RNA viruses is the initiation of RNA synthesis from the 3' terminus of the template by a de novo mechanism. This dissertation research is focused on the characterization of the interactions between the HCV RdRp and ligands (or proteins) that are involved during de novo initiation of RNA synthesis by the HCV RdRp.

ISBN: 9780542504754Subjects--Topical Terms:

1017722
Chemistry, Biochemistry.

Protein-ligand and protein-protein interactions involved in de novo initiation of RNA synthesis by the hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp).
LDR:03547nmm 2200325 4500 001 1820300
005 20061023071459.5
008 130610s2006 eng d
020 $a 9780542504754
035 $a (UnM)AAI3204540
035 $a AAI3204540
040 $a UnM $c UnM
100 1 $a Kim, Young-Chan. $3 1909531
245 1 0 $a Protein-ligand and protein-protein interactions involved in de novo initiation of RNA synthesis by the hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp).
300 $a 136 p.
500 $a Source: Dissertation Abstracts International, Volume: 67-01, Section: B, page: 0249.
500 $a Adviser: C. Cheng Kao.
502 $a Thesis (Ph.D.)--Indiana University, 2006.
520 $a Hepatitis C Virus (HCV) is a major etiological agent of non-A, non-B chronic hepatitis. An estimated 180 million people worldwide are infected with this virus. Given this huge epidemic and relative low efficacy of current treatment for hepatitis C, it is urgent to develop better antiviral therapeutics. Better understanding of the mechanism of all aspect of RNA-dependent RNA synthesis will contributes for this purpose. A feature unique to RNA viruses is the initiation of RNA synthesis from the 3' terminus of the template by a de novo mechanism. This dissertation research is focused on the characterization of the interactions between the HCV RdRp and ligands (or proteins) that are involved during de novo initiation of RNA synthesis by the HCV RdRp.
520 $a Fluorescence spectroscopy was used to examine the equilibrium binding of ligands by the HCV RdRp. Three independent intrinsic and extrinsic fluorophores were used to characterize protein-ligand and protein-protein interactions. Using these fluorescence assays, we determined that the HCV RdRp had an approximately 9-fold higher affinity for Mn2+ than for Mg2+. Furthermore, the minimal length of RNA required for stable binding to the HCV RdRp was determined to be seven nucleotides. HCV RdRp bound to de novo initiation competent RNA with &sim;2 fold enhanced affinity in the presence of initiation specific GTP and Mn2+ ion, suggesting a modest stabilization effect exists during the formation of a productive initiation ternary complex.
520 $a Mapping of the RNA contact sites on the HCV RdRp were performed by using reversible formaldehyde cross-linking coupled with RNA affinity chromatography, and mass spectrometric analyses. Identified cross-linked peptides are located in the fingers subdomain, connecting loops of fingers and thumb subdomains and active site cavity. The identified regions contained all predicted RNA contacting residues from the crystal structures. Mutational analysis of the HCV RdRp at several likely residues presumed to be important in contacting RNA decreased RNA synthesis in vitro. When the HCV replicons containing the mutations were tested in cultured liver cells, all but one mutation prevented the replication, indicating that the sites mapped are important for HCV RNA replication.
520 $a The interaction between the HCV RdRp with itself and with the HCV NS3 protein was also examined using biochemical and biophysical approaches. Altogether, this work contributes to understanding of mechanism of RNA synthesis by the HCV RdRp.
590 $a School code: 0093.
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
690 $a 0487
690 $a 0410
690 $a 0307
710 2 0 $a Indiana University. $3 960096
773 0 $t Dissertation Abstracts International $g 67-01B.
790 1 0 $a Kao, C. Cheng, $e advisor
790 $a 0093
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3204540