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Retroviral RNA nuclear export elemen...

Swanson, Chad Michael.

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Retroviral RNA nuclear export elements regulate protein function and viral assembly.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Retroviral RNA nuclear export elements regulate protein function and viral assembly./
作者:	Swanson, Chad Michael.
面頁冊數:	181 p.
附註:	Source: Dissertation Abstracts International, Volume: 65-11, Section: B, page: 5539.
Contained By:	Dissertation Abstracts International65-11B.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3152107
ISBN:	0496125079

Retroviral RNA nuclear export elements regulate protein function and viral assembly.
Swanson, Chad Michael.

Retroviral RNA nuclear export elements regulate protein function and viral assembly. - 181 p.

Source: Dissertation Abstracts International, Volume: 65-11, Section: B, page: 5539.

Thesis (Ph.D.)--University of Pennsylvania, 2004.

Recently, it has been recognized that mRNA-associated events occurring in the nucleus can regulate the fate of the mRNA in the cytosol. We address the hypothesis that the nuclear history of HIV gag-pol RNA can alter the activity of the encoded protein. In human cells, HIV unspliced RNA normally uses the Crm1 nuclear export pathway in an RRE/Rev-dependent manner. Routing gag containing RNA nuclear export to another pathway disrupts Gag assembly and particle production for several independent constructs. However, in the context of four tandem copies of the M-PMV CTE (4 x CTE), gag-pol RNA using the NXF1 nuclear export pathway is translated into Gag protein that can productively assemble. Murine cells are notable for their inability to support normal assembly of HIV particles and we show that altering the RNA nuclear export element used by HIV gag-pol mRNA from the RRE to the 4 x CTE restores both the trafficking of Gag to cellular membranes and efficient particle production in these cells. These results suggest that two phases of the HIV life cycle, RNA export and capsid assembly, that have hitherto been regarded as distinct are, in fact, linked. Thus, protein function and fate may depend upon the full and precise history of its encoding mRNA.

ISBN: 0496125079Subjects--Topical Terms:

1017734
Biology, Microbiology.

Retroviral RNA nuclear export elements regulate protein function and viral assembly.
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245 1 0 $a Retroviral RNA nuclear export elements regulate protein function and viral assembly.
300 $a 181 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-11, Section: B, page: 5539.
500 $a Supervisor: Michael H. Malim.
502 $a Thesis (Ph.D.)--University of Pennsylvania, 2004.
520 $a Recently, it has been recognized that mRNA-associated events occurring in the nucleus can regulate the fate of the mRNA in the cytosol. We address the hypothesis that the nuclear history of HIV gag-pol RNA can alter the activity of the encoded protein. In human cells, HIV unspliced RNA normally uses the Crm1 nuclear export pathway in an RRE/Rev-dependent manner. Routing gag containing RNA nuclear export to another pathway disrupts Gag assembly and particle production for several independent constructs. However, in the context of four tandem copies of the M-PMV CTE (4 x CTE), gag-pol RNA using the NXF1 nuclear export pathway is translated into Gag protein that can productively assemble. Murine cells are notable for their inability to support normal assembly of HIV particles and we show that altering the RNA nuclear export element used by HIV gag-pol mRNA from the RRE to the 4 x CTE restores both the trafficking of Gag to cellular membranes and efficient particle production in these cells. These results suggest that two phases of the HIV life cycle, RNA export and capsid assembly, that have hitherto been regarded as distinct are, in fact, linked. Thus, protein function and fate may depend upon the full and precise history of its encoding mRNA.
590 $a School code: 0175.
650 4 $a Biology, Microbiology. $3 1017734
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791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3152107