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Amperometric DNA sensing using wired...

Zhang, Yongchao.

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Amperometric DNA sensing using wired enzyme based electrodes.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Amperometric DNA sensing using wired enzyme based electrodes./
Author:	Zhang, Yongchao.
Description:	167 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-02, Section: B, page: 0712.
Contained By:	Dissertation Abstracts International65-02B.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3122806
ISBN:	0496701347

Amperometric DNA sensing using wired enzyme based electrodes.
Zhang, Yongchao.

Amperometric DNA sensing using wired enzyme based electrodes. - 167 p.

Source: Dissertation Abstracts International, Volume: 65-02, Section: B, page: 0712.

Thesis (Ph.D.)--The University of Texas at Austin, 2003.

A water soluble copolymer of acrylamide and 4-vinylpyridine complexed with [Os(bpy)2Cl]+/2+ (bpy = 2,2'-bipyridine), was synthesized. An electrodeposition method of making redox polymer films on electrodes was developed. This method was also shown to be effective in incorporating enzymes and amine-terminated DNA sequences in the redox polymer film.

ISBN: 0496701347Subjects--Topical Terms:

586156
Chemistry, Analytical.

Amperometric DNA sensing using wired enzyme based electrodes.
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100 1 $a Zhang, Yongchao. $3 1907501
245 1 0 $a Amperometric DNA sensing using wired enzyme based electrodes.
300 $a 167 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-02, Section: B, page: 0712.
500 $a Supervisor: Adam Heller.
502 $a Thesis (Ph.D.)--The University of Texas at Austin, 2003.
520 $a A water soluble copolymer of acrylamide and 4-vinylpyridine complexed with [Os(bpy)2Cl]+/2+ (bpy = 2,2'-bipyridine), was synthesized. An electrodeposition method of making redox polymer films on electrodes was developed. This method was also shown to be effective in incorporating enzymes and amine-terminated DNA sequences in the redox polymer film.
520 $a A 38-base DNA sequence was detected at 20 pM concentration in 15--35 muL droplets by an electrochemical enzyme-amplified sandwich-type assay on a mass-manufacturable screen printed carbon electrode with a diameter of 3.5 mm. A DNA-capturing oligonucleotide was attached to the pre-deposited redox polymer film using the electrodeposition method. The electrode was exposed to the droplet containing the tested DNA sample, and was then treated with a droplet containing horseradish peroxidase-labeled detection sequence. Formation of the capture-target-detection sandwich brought the horseradish peroxidase-label of the detection sequence in electrical contact with the redox polymer, making the sandwich an electrocatalyst for the reduction of hydrogen peroxide to water at +0.2 V (Ag/AgCl).
520 $a The radial diffusion of electrons through the redox polymer film on the microelectrode allowed the electrodeposition of a thicker film of the redox polymer, an increase in the loading of the capture sequence, and increased the collection efficiency of the electron vacancies originating in the electroreduced H2O2. With a 10-mum diameter carbon fiber microelectrode, as few as 3000 copies of the 38-basse DNA sequence were detected at 0.5 fM concentration in a 10 muL sample.
520 $a A biofuel cell operating at a power density of 50 muW cm-2 at 0.5 V under physiological conditions (air saturated, pH 7.4, 0.14 M NaCl, 37.5°C, 15 mM glucose) was developed. The cell had a glucose electro-oxidizing anode and an O2 electro-reducing cathode. The anode and the cathode were 7 mum diameter, 2 cm long carbon fibers, on which the catalytic enzyme-redox polymer adducts were cross-linked. When the miniature cell operated at 0.5 V, the power output dropped to about 60% of its initial value after 2 days of continuous operation at 37.5°C.
590 $a School code: 0227.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Engineering, Chemical. $3 1018531
690 $a 0486
690 $a 0542
710 2 0 $a The University of Texas at Austin. $3 718984
773 0 $t Dissertation Abstracts International $g 65-02B.
790 1 0 $a Heller, Adam, $e advisor
790 $a 0227
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3122806