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The molecular biology of orchids: Tr...

Saxon, Herbert Leslie.

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The molecular biology of orchids: Transformation by Agrobacterium tumefaciens and DNA fingerprinting.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The molecular biology of orchids: Transformation by Agrobacterium tumefaciens and DNA fingerprinting./
作者:	Saxon, Herbert Leslie.
面頁冊數:	53 p.
附註:	Source: Dissertation Abstracts International, Volume: 56-05, Section: B, page: 2477.
Contained By:	Dissertation Abstracts International56-05B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9528072

The molecular biology of orchids: Transformation by Agrobacterium tumefaciens and DNA fingerprinting.
Saxon, Herbert Leslie.

The molecular biology of orchids: Transformation by Agrobacterium tumefaciens and DNA fingerprinting. - 53 p.

Source: Dissertation Abstracts International, Volume: 56-05, Section: B, page: 2477.

Thesis (D.Ed.)--Ball State University, 1995.

The work reported here was done at the Wheeler Orchid Collection and Species Bank and the Department of Biology at Ball State University. We have developed a research teaching program with two applied research goals: genetically transforming and DNA fingerprinting orchid tissue. As part of their molecular biology education, students have investigated the genetic transformation of orchids for mitigating viral symptoms and the identification of unknown orchids by DNA fingerprinting. In a second application of the technology, DNA fingerprinting has been used to determine evolutionary relationships and to quantify genetic diversity among orchids.Subjects--Topical Terms:

1017719
Biology, Molecular.

The molecular biology of orchids: Transformation by Agrobacterium tumefaciens and DNA fingerprinting.
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100 1 $a Saxon, Herbert Leslie. $3 1907479
245 1 4 $a The molecular biology of orchids: Transformation by Agrobacterium tumefaciens and DNA fingerprinting.
300 $a 53 p.
500 $a Source: Dissertation Abstracts International, Volume: 56-05, Section: B, page: 2477.
500 $a Adviser: Carolyn Vann.
502 $a Thesis (D.Ed.)--Ball State University, 1995.
520 $a The work reported here was done at the Wheeler Orchid Collection and Species Bank and the Department of Biology at Ball State University. We have developed a research teaching program with two applied research goals: genetically transforming and DNA fingerprinting orchid tissue. As part of their molecular biology education, students have investigated the genetic transformation of orchids for mitigating viral symptoms and the identification of unknown orchids by DNA fingerprinting. In a second application of the technology, DNA fingerprinting has been used to determine evolutionary relationships and to quantify genetic diversity among orchids.
520 $a This dissertation details the background and need for this project and the research that was done to start it. As the early work has developed and students have added their contributions, the data have developed into two papers formatted for submission to scientific journals. They are included as results.
520 $a The first is a project designed to insert exognenous DNA into orchid tissue. The soil microbe Agrobacterium tumefaciens causes crown-gall tumors to develop in its plant hosts by inserting DNA into their cells which then controls the biosynthesis of development-controlling hormones. A. tumefaciens which has been disarmed has been routinely used to bioengineer dicotyledonous plants but its use has been rare on monocotyledons. In this paper, we report that A. tumefaciens transformed embryonic orchid tissue and caused alteration in its normal developmental course.
520 $a The second paper details the DNA fingerprinting of tissue from Aplectrum hymale, a terrestrial orchid native to this climate. Three populations of A. hymale have been sampled and DNA extracted from the tissue samples. RAPD primers were used to prime PCR amplifications of random sequences of the DNA and the amplified DNA was visualized by gel electrophoresis. Loci of the resulting bands were treated as potentially multiallelic gene loci and heterozygosity between and within subpopulations was calculated. We report that the three populations could be partially differentiated by this procedure and that the two populations located nearest to each other yielded the least between-subpopulations heterozygosity. We report very high levels of genetic diversity between individuals within small subpopulations in spite of the fact that these subpopulations are considered to be primarily clonal in reproductive nature.
590 $a School code: 0013.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Botany. $3 1017825
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710 2 0 $a Ball State University. $3 1018080
773 0 $t Dissertation Abstracts International $g 56-05B.
790 1 0 $a Vann, Carolyn, $e advisor
790 $a 0013
791 $a D.Ed.
792 $a 1995
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9528072