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Wakefulness and sleep: Intrinsic mo...

Decker, Michael John.

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Wakefulness and sleep: Intrinsic modulators of the suprachiasmatic nucleus.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Wakefulness and sleep: Intrinsic modulators of the suprachiasmatic nucleus./
作者:	Decker, Michael John.
面頁冊數:	113 p.
附註:	Source: Dissertation Abstracts International, Volume: 60-11, Section: B, page: 5371.
Contained By:	Dissertation Abstracts International60-11B.
標題:	Biology, Neuroscience. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9949265
ISBN:	0599524243

Wakefulness and sleep: Intrinsic modulators of the suprachiasmatic nucleus.
Decker, Michael John.

Wakefulness and sleep: Intrinsic modulators of the suprachiasmatic nucleus. - 113 p.

Source: Dissertation Abstracts International, Volume: 60-11, Section: B, page: 5371.

Thesis (Ph.D.)--Case Western Reserve University (Health Sciences), 1999.

Little doubt exists regarding the relationship between the behavioral states of wakefulness and sleep with the environmental light-dark cycle. Yet, the influence of these behavioral states upon the Suprachiasmatic Nucleus (SCN), the circadian pacemaker, are not well described. This research was designed to (a) evaluate the effect of acute changes in environmental illumination upon sleep-wake architecture, and (b) determine the effects of sleep and wakefulness upon the functional responsiveness of the SCN to photic stimulation. Thirty-two Harlan male rats were instrumented for chronic electroencephalography and housed under a 12:12 hour light-dark cycle. Following recovery from surgery, animals were connected to a polygraphic recorder and placed into a sealed, light and temperature regulated, environmental cubicle. At half-hour intervals, an automatic timer turned off the light source and exposed the animals to 5 minutes of darkness. The light was then automatically turned light on following 5 minutes of darkness. This continued until 1500 hours at which time animals were randomly assigned to spend the final hour of the study (1500--1600) as either awake in the light or in the dark, or left alone to permit sleep in either the light or the dark. Following this, animals were sacrificed and perfused, and the brain was removed and immunohistochemically processed for Cfos protein expression. Findings from this study suggest that the manner in which animals responded to abrupt changes in environmental illumination were dependent upon ambient light intensity, prior behavioral state, and strain. The number (x¯ +/- one standard deviation) of Cfos positive neurons in the SCN of animals kept awake in the fight were significantly greater (p < 0.05) than were numbers from animals kept awake in the dark. In contrast, there was no difference between the number of Cfos positive neurons found in animals permitted to sleep in the fight compared with those permitted to sleep in the dark. Further, among sleeping animals, the occurrence of paradoxical sleep was associated with reduced numbers of Cfos positive neurons (Rsq = 0.41, p = 0.03), a relationship independent of the presence or absence of light. These findings suggest that behavioral state is a significant intrinsic modulator of the SCN.

ISBN: 0599524243Subjects--Topical Terms:

1017680
Biology, Neuroscience.

Wakefulness and sleep: Intrinsic modulators of the suprachiasmatic nucleus.
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