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Hormone mediated transport of calciu...

Sterling, Tremaine M.

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Hormone mediated transport of calcium and phosphate in polarized epithelial cells.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Hormone mediated transport of calcium and phosphate in polarized epithelial cells./
作者:	Sterling, Tremaine M.
面頁冊數:	73 p.
附註:	Source: Masters Abstracts International, Volume: 42-06, page: 2172.
Contained By:	Masters Abstracts International42-06.
標題:	Health Sciences, Nutrition. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1419908
ISBN:	0496246607

Hormone mediated transport of calcium and phosphate in polarized epithelial cells.
Sterling, Tremaine M.

Hormone mediated transport of calcium and phosphate in polarized epithelial cells. - 73 p.

Source: Masters Abstracts International, Volume: 42-06, page: 2172.

Thesis (M.S.)--Utah State University, 2004.

The effects of 1,25(OH)2D3, PTH and 25(OH)D 3 on phosphate or calcium uptake were studied in cultured chick enterocytes using a period of 10 min after hormone addition. Cells treated with 130 pM 1,25(OH)2D3 showed an increase in 32P uptake. Similar studies with 65 pM bPTH(1-34) or 100nm 25(OH)D 3 resulted in increased 45Ca uptake. Confocal microscopy using the endocytotic marker dye, FM 1-43, revealed that membrane trafficking is rapid enough to contribute to ion transport.

ISBN: 0496246607Subjects--Topical Terms:

1017801
Health Sciences, Nutrition.

Hormone mediated transport of calcium and phosphate in polarized epithelial cells.
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500 $a Major Professor: Ilka Nemere.
502 $a Thesis (M.S.)--Utah State University, 2004.
520 $a The effects of 1,25(OH)2D3, PTH and 25(OH)D 3 on phosphate or calcium uptake were studied in cultured chick enterocytes using a period of 10 min after hormone addition. Cells treated with 130 pM 1,25(OH)2D3 showed an increase in 32P uptake. Similar studies with 65 pM bPTH(1-34) or 100nm 25(OH)D 3 resulted in increased 45Ca uptake. Confocal microscopy using the endocytotic marker dye, FM 1-43, revealed that membrane trafficking is rapid enough to contribute to ion transport.
520 $a Studies were undertaken using the PKC and PKA inhibitors, chelerythrine and Rp-cAMP, respectively. Results with 1,25(OH)2D3 and these inhibitors were complex, whereas preincubation of enterocytes with Ab099 (against a membrane receptor for the seco-steroid) abolished 1,25(OH) 2D3-stimulated 32P uptake, and FM 1-43 redistribution. The stimulatory actions of bPTH(1-34) or 25(OH)D3 on 45 Ca uptake and membrane trafficking were decreased by Rp-cAMP.
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