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Functional characterization of N-gen...

Yamamoto, Miki Lynn.

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Functional characterization of N-gene mediated signaling leading to TMV resistance in solanaceous species.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Functional characterization of N-gene mediated signaling leading to TMV resistance in solanaceous species./
作者:	Yamamoto, Miki Lynn.
面頁冊數:	138 p.
附註:	Source: Dissertation Abstracts International, Volume: 66-08, Section: B, page: 4096.
Contained By:	Dissertation Abstracts International66-08B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3187189
ISBN:	054229169X

Functional characterization of N-gene mediated signaling leading to TMV resistance in solanaceous species.
Yamamoto, Miki Lynn.

Functional characterization of N-gene mediated signaling leading to TMV resistance in solanaceous species. - 138 p.

Source: Dissertation Abstracts International, Volume: 66-08, Section: B, page: 4096.

Thesis (Ph.D.)--University of California, Berkeley, 2005.

Plants possess natural preformed mechanisms for defense against pathogens. Gene-for-gene resistance is an effective resistance strategy in which a single dominant plant gene (R) recognizes a pathogen-derived molecule (avr) and triggers a set of defense responses leading to resistance. Following the gene-for-gene model, the N gene from Nicotiana glutinosa recognizes a 50kDa portion of the TMV replicase and activates resistance to the tobacco mosaic virus (TMV). N confers TMV resistance in most Solanaceae plant species, including tobacco, a wild relative of tobacco Nicotiana benthamiana, and tomato. The N-suppressor mutant sun1-1, deficient in the tomato homolog of Arabidopsis EDS1, shows R gene suppression and enhanced susceptibility to TMV and other pathogens. Availability of mutant sun1-1 provides a unique opportunity to further assess N gene signaling and shared R gene signaling pathways in Solanaceae.

ISBN: 054229169XSubjects--Topical Terms:

1017719
Biology, Molecular.

Functional characterization of N-gene mediated signaling leading to TMV resistance in solanaceous species.
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245 1 0 $a Functional characterization of N-gene mediated signaling leading to TMV resistance in solanaceous species.
300 $a 138 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-08, Section: B, page: 4096.
500 $a Chairs: Barbara Baker; Peter Quail.
502 $a Thesis (Ph.D.)--University of California, Berkeley, 2005.
520 $a Plants possess natural preformed mechanisms for defense against pathogens. Gene-for-gene resistance is an effective resistance strategy in which a single dominant plant gene (R) recognizes a pathogen-derived molecule (avr) and triggers a set of defense responses leading to resistance. Following the gene-for-gene model, the N gene from Nicotiana glutinosa recognizes a 50kDa portion of the TMV replicase and activates resistance to the tobacco mosaic virus (TMV). N confers TMV resistance in most Solanaceae plant species, including tobacco, a wild relative of tobacco Nicotiana benthamiana, and tomato. The N-suppressor mutant sun1-1, deficient in the tomato homolog of Arabidopsis EDS1, shows R gene suppression and enhanced susceptibility to TMV and other pathogens. Availability of mutant sun1-1 provides a unique opportunity to further assess N gene signaling and shared R gene signaling pathways in Solanaceae.
520 $a This dissertation reports functional characterization of early transcriptional and signaling events in the resistance response to TMV in tomato, and the establishment of a transient overexpression system to assess N protein function in planta. Gene expression in three tomato genotypes, wildtype ( N) resistant, wildtype (n) susceptible, and enhanced susceptible sun1-1 mutant tomato, was assessed after TMV challenge. Transcriptional control of N was quantitatively characterized after TMV challenge. Total N transcript and the N alternative transcript were induced after TMV challenge in resistant tomato. TMV-altered N gene expression and alternative splicing were not affected by TMV challenge in the sun1-1 mutant. Cellular genes displaying TMV-altered gene expression were identified in cDNA microarray analyses of the three genotypes at three time points after TMV challenge. Changes in gene-expression level were confirmed using quantitative Real-time PCR, and selected genes were assessed for function in the N-resistance pathway. A bZIP transcription factor TGA2.1 was found to play a role in the N-mediated TMV resistance and Bs2/avrBs2-mediated cell death. Lastly, a transient overexpression system was established to detect N protein in tobacco and to allow future assessment of interactions with other cellular components in planta.
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773 0 $t Dissertation Abstracts International $g 66-08B.
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790 1 0 $a Quail, Peter, $e advisor
790 $a 0028
791 $a Ph.D.
792 $a 2005
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