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The roles of messenger RNA synthesis...

Cleary, Michael David.

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The roles of messenger RNA synthesis and messenger RNA decay in the regulation of gene expression during Toxoplasma gondii asexual development.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The roles of messenger RNA synthesis and messenger RNA decay in the regulation of gene expression during Toxoplasma gondii asexual development./
作者:	Cleary, Michael David.
面頁冊數:	199 p.
附註:	Source: Dissertation Abstracts International, Volume: 65-11, Section: B, page: 5532.
Contained By:	Dissertation Abstracts International65-11B.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3153200
ISBN:	0496136143

The roles of messenger RNA synthesis and messenger RNA decay in the regulation of gene expression during Toxoplasma gondii asexual development.
Cleary, Michael David.

The roles of messenger RNA synthesis and messenger RNA decay in the regulation of gene expression during Toxoplasma gondii asexual development. - 199 p.

Source: Dissertation Abstracts International, Volume: 65-11, Section: B, page: 5532.

Thesis (Ph.D.)--Stanford University, 2005.

Asexual development in Toxoplasma gondii is a vital aspect of the parasite's lifecycle, allowing transmission and avoidance of the host immune response. Development of rapidly dividing tachyzoites into slowly growing, encysted bradyzoites involves significant changes in both physiology and morphology. The identification of developmentally-regulated genes is important for understanding the biological differences between tachyzoites and bradyzoites.

ISBN: 0496136143Subjects--Topical Terms:

1017734
Biology, Microbiology.

The roles of messenger RNA synthesis and messenger RNA decay in the regulation of gene expression during Toxoplasma gondii asexual development.
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245 1 4 $a The roles of messenger RNA synthesis and messenger RNA decay in the regulation of gene expression during Toxoplasma gondii asexual development.
300 $a 199 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-11, Section: B, page: 5532.
500 $a Adviser: John Boothroyd.
502 $a Thesis (Ph.D.)--Stanford University, 2005.
520 $a Asexual development in Toxoplasma gondii is a vital aspect of the parasite's lifecycle, allowing transmission and avoidance of the host immune response. Development of rapidly dividing tachyzoites into slowly growing, encysted bradyzoites involves significant changes in both physiology and morphology. The identification of developmentally-regulated genes is important for understanding the biological differences between tachyzoites and bradyzoites.
520 $a In this study, microarray analysis was used to study gene expression during bradyzoite development. To compare mRNA transcript abundance between tachyzoites and bradyzoites, a novel method for measuring mRNA abundance on cDNA microarrays (which uses a vector-derived reference probe) was developed. These experiments identified many novel developmentally-regulated genes and revealed previously unknown aspects of bradyzoite biology.
520 $a To identify the mechanisms responsible for changes in gene expression, a novel method to measure mRNA synthesis and mRNA decay on microarrays was developed. This method uses thio-containing uracil (sU) to biosynthetically label RNA via the uracil phosphoribosyltransferase (UPRT) enzyme of T. gondii. Characterization of this technique and its application to the study of mRNA synthesis and decay in tachyzoites and bradyzoites is the main focus of this dissertation. These studies identified distinct mechanisms responsible for the regulation of gene expression during bradyzoite development. Preliminary experiments testing the effect of 3' UTR sequences on mRNA stability were also performed.
520 $a RNA labeling via UPRT could allow the same types of studies described for T. gondii to be performed in other organisms. Experiments are presented that show that the endogenous UPRT of Saccharomyces cerevisiae can be used for sU labeling of RNA. Mammals lack UPRT but transgenic HeLa cells that express T. gondii UPRT can incorporate sU into RNA. Finally, specific in vivo labeling of RNA in UPRT expressing cells is demonstrated by selective labeling of T. gondii RNA during a mouse infection. These preliminary results suggest that transgenic expression of T. gondii UPRT in other organism may be particularly useful for cell-specific analysis of gene expression in vivo.
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791 $a Ph.D.
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