東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

The comparison of two hyphenated tec...

Krisko, Ryan Matthew.

Linked to FindBook

Google Book

Amazon

博客來

The comparison of two hyphenated techniques, LC/MS and CE/MS, for bioanalytical challenges.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The comparison of two hyphenated techniques, LC/MS and CE/MS, for bioanalytical challenges./
Author:	Krisko, Ryan Matthew.
Description:	155 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-08, Section: B, page: 3997.
Contained By:	Dissertation Abstracts International65-08B.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3141477
ISBN:	0496886096

The comparison of two hyphenated techniques, LC/MS and CE/MS, for bioanalytical challenges.
Krisko, Ryan Matthew.

The comparison of two hyphenated techniques, LC/MS and CE/MS, for bioanalytical challenges. - 155 p.

Source: Dissertation Abstracts International, Volume: 65-08, Section: B, page: 3997.

Thesis (Ph.D.)--The University of Kansas, 2004.

Hyphenated techniques using mass spectrometry as a detection strategy have become some of the most powerful analytical methodologies for bioanalytical challenges. Particularly, high performance liquid chromatography with mass spectrometric detection (LC/MS) and gas chromatography with mass spectrometric detection (GC/MS) are techniques that have gained widespread acceptance while capillary electrophoresis with mass spectrometric detection (CE/MS) remains in its infancy. Even though CE/MS has the capability of achieving better separation efficiencies and requires very low sample volumes for analysis than LC/MS, it lacks both the robustness and low concentration limits of detection (LOD) with respect to LC/MS and thus remains a less accepted analytical method.

ISBN: 0496886096Subjects--Topical Terms:

586156
Chemistry, Analytical.

The comparison of two hyphenated techniques, LC/MS and CE/MS, for bioanalytical challenges.
LDR:03286nmm 2200313 4500 001 1815619
005 20060710080743.5
008 130610s2004 eng d
020 $a 0496886096
035 $a (UnM)AAI3141477
035 $a AAI3141477
040 $a UnM $c UnM
100 1 $a Krisko, Ryan Matthew. $3 1905035
245 1 4 $a The comparison of two hyphenated techniques, LC/MS and CE/MS, for bioanalytical challenges.
300 $a 155 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-08, Section: B, page: 3997.
500 $a Adviser: Craig Lunte.
502 $a Thesis (Ph.D.)--The University of Kansas, 2004.
520 $a Hyphenated techniques using mass spectrometry as a detection strategy have become some of the most powerful analytical methodologies for bioanalytical challenges. Particularly, high performance liquid chromatography with mass spectrometric detection (LC/MS) and gas chromatography with mass spectrometric detection (GC/MS) are techniques that have gained widespread acceptance while capillary electrophoresis with mass spectrometric detection (CE/MS) remains in its infancy. Even though CE/MS has the capability of achieving better separation efficiencies and requires very low sample volumes for analysis than LC/MS, it lacks both the robustness and low concentration limits of detection (LOD) with respect to LC/MS and thus remains a less accepted analytical method.
520 $a The purpose of this research was to construct a simple CE/MS interface and attempt to mitigate the LOD and robustness limitations that the technique suffers. After the CE/MS interface was constructed, experiments were performed involving several bioanalytical challenges to explore the utility of CE/MS and LC/MS. This also served as a means to compare the relative advantages and disadvantages of the two techniques in a side by side comparison.
520 $a The first study was the analysis of bupivacaine metabolites in rat urine using both LC/MS and CE/MS to obtain structural information about the different metabolites. From the CE/MS and LC/MS data, structural information about nine different metabolites was obtained. A comparison of the two techniques showed that LC/MS was able to detect metabolites below the LOD of CE/MS. However, it was demonstrated that CE/MS was more efficient in separating the analytes from the background matrix components.
520 $a The second study was to quantitate the biomarker, 8-hydroxy-2 '-deoxyguanosine, in urine using both CE/MS and LC/MS. Using tandem mass spectrometry and HPLC (LC/MS/MS), the quantitation of 8-OHdG in urine was possible. CE/MS was unable to detect 8-OHdG in urine due to insufficient LOD.
520 $a The last application consisted of using LC/MS for the fast development of HPLC methods. By combining LC/MS with DryLab 2000, it was possible to quickly develop HPLC methods on several samples. This is of particular interest to the pharmaceutical industry since the strategy reduces the amount of time and resources needed to develop HPLC methods.
590 $a School code: 0099.
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0486
710 2 0 $a The University of Kansas. $3 1288487
773 0 $t Dissertation Abstracts International $g 65-08B.
790 1 0 $a Lunte, Craig, $e advisor
790 $a 0099
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3141477