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Bacterial oxidation of arsenite at H...

Salmassi, Tina Megerdichian.

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Bacterial oxidation of arsenite at Hot Creek: Characterization of biofilm communities and isolation of novel bacteria associated with aquatic macrophytes (California).

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Bacterial oxidation of arsenite at Hot Creek: Characterization of biofilm communities and isolation of novel bacteria associated with aquatic macrophytes (California)./
作者:	Salmassi, Tina Megerdichian.
面頁冊數:	226 p.
附註:	Source: Dissertation Abstracts International, Volume: 62-04, Section: B, page: 1716.
Contained By:	Dissertation Abstracts International62-04B.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3011604
ISBN:	0493209492

Bacterial oxidation of arsenite at Hot Creek: Characterization of biofilm communities and isolation of novel bacteria associated with aquatic macrophytes (California).
Salmassi, Tina Megerdichian.

Bacterial oxidation of arsenite at Hot Creek: Characterization of biofilm communities and isolation of novel bacteria associated with aquatic macrophytes (California). - 226 p.

Source: Dissertation Abstracts International, Volume: 62-04, Section: B, page: 1716.

Thesis (Ph.D.)--California Institute of Technology, 2001.

Hot Creek, a tributary of the Owens River in the Long Valley Caldera in California, contains naturally elevated concentrations of arsenic as a result of geothermal activity. This site is of particular interest because of its substantial impact on the quality of the drinking water supply for the City of Los Angeles.

ISBN: 0493209492Subjects--Topical Terms:

1017734
Biology, Microbiology.

Bacterial oxidation of arsenite at Hot Creek: Characterization of biofilm communities and isolation of novel bacteria associated with aquatic macrophytes (California).
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245 1 0 $a Bacterial oxidation of arsenite at Hot Creek: Characterization of biofilm communities and isolation of novel bacteria associated with aquatic macrophytes (California).
300 $a 226 p.
500 $a Source: Dissertation Abstracts International, Volume: 62-04, Section: B, page: 1716.
500 $a Adviser: Janet G. Hering.
502 $a Thesis (Ph.D.)--California Institute of Technology, 2001.
520 $a Hot Creek, a tributary of the Owens River in the Long Valley Caldera in California, contains naturally elevated concentrations of arsenic as a result of geothermal activity. This site is of particular interest because of its substantial impact on the quality of the drinking water supply for the City of Los Angeles.
520 $a Previous studies revealed a rapid in situ oxidation of arsenite in Hot Creek. In this work, bacterial oxidation of arsenite is viewed from the perspective of cultivation-based studies (focusing on pure cultures of arsenite oxidizers) and molecular technique-based studies (surveying the community under ambient conditions).
520 $a The cultivation-based techniques yielded four new arsenite oxidizers. One isolate, Agrobacterium albertimagni strain AOL15, is an alpha-proteobacterium that was isolated using an enrichment-based isolation technique with arsenite concentrations much higher than ambient levels. Values of the kinetic parameters Ks(3.4 +/- 2.2 muM) and V max (1.81 +/- 0.58 x 10-12 mumole·cell -1·min-1) were determined for AOL15. The Ks is near the ambient concentration of arsenite in Hot Creek. However, molecular-based techniques suggested that AOL15 is not a significant member of the biofilm associated with submerged macrophytes.
520 $a The other three oxidizers, YED1--18, YED6--4, and YED6--21, all beta-proteobacteria of the genus Hydrogenophaga, were isolated from solid media. Molecular techniques suggested that beta-proteobacteria are important members of the macrophyte surface community. In the molecular survey, one sequence from Clone #44 (partial) was found to be 99.6% identical to YED6--21. This result, coupled with the isolation of the Hydrogenophaga oxidizers from a million-fold dilution of a suspension of cells from the macrophyte surface, suggests that the Hydrogenophaga are significant members of this community and may be the dominant arsenite oxidizers.
520 $a The densities of total and oxidizer cells associated with the submerged macrophytes in Hot Creek were estimated using most probable number (MIPN) analysis. The normalized MEPN values were 1.4 x 109 total cells/g dry wt. plant and 3.3 x 108 oxidizer cells/g dry wt. plant. These estimates suggest that the oxidizers constitute a significant fraction (on average 24%) of the overall biofilm community.
590 $a School code: 0037.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Environmental Sciences. $3 676987
650 4 $a Biogeochemistry. $3 545717
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710 2 0 $a California Institute of Technology. $3 726902
773 0 $t Dissertation Abstracts International $g 62-04B.
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791 $a Ph.D.
792 $a 2001
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