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Optimization of the fermentation pro...

Simeone, Jeffrey Michael.

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Optimization of the fermentation process for Escherichia coli strain GI724.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Optimization of the fermentation process for Escherichia coli strain GI724./
作者:	Simeone, Jeffrey Michael.
面頁冊數:	78 p.
附註:	Source: Masters Abstracts International, Volume: 43-05, page: 1768.
Contained By:	Masters Abstracts International43-05.
標題:	Engineering, Chemical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1425526
ISBN:	0496987550

Optimization of the fermentation process for Escherichia coli strain GI724.
Simeone, Jeffrey Michael.

Optimization of the fermentation process for Escherichia coli strain GI724. - 78 p.

Source: Masters Abstracts International, Volume: 43-05, page: 1768.

Thesis (M.S.Eng.)--University of Massachusetts Lowell, 2005.

This thesis focuses on the optimization of the fermentation of E. coli strain GI724. This strain has been genetically altered and contains the cellular coding for the production of Troponin I, a protein used in muscle contraction and has been shown to inhibit the growth of blood vessels in tumors. The optimization included not only the examination of achieving a high cell density but also a high expression level for the protein of interest. The optimization included ascertaining most of the key nutrients for this strain, determining the point at which some nutrients become deficient, examination of the relationship between glycerol concentrations and acetate production by the cell, and the effect of the acetate level on the cell growth rate.

ISBN: 0496987550Subjects--Topical Terms:

1018531
Engineering, Chemical.

Optimization of the fermentation process for Escherichia coli strain GI724.
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100 1 $a Simeone, Jeffrey Michael. $3 1904559
245 1 0 $a Optimization of the fermentation process for Escherichia coli strain GI724.
300 $a 78 p.
500 $a Source: Masters Abstracts International, Volume: 43-05, page: 1768.
500 $a Supervisor: Carl Lawton.
502 $a Thesis (M.S.Eng.)--University of Massachusetts Lowell, 2005.
520 $a This thesis focuses on the optimization of the fermentation of E. coli strain GI724. This strain has been genetically altered and contains the cellular coding for the production of Troponin I, a protein used in muscle contraction and has been shown to inhibit the growth of blood vessels in tumors. The optimization included not only the examination of achieving a high cell density but also a high expression level for the protein of interest. The optimization included ascertaining most of the key nutrients for this strain, determining the point at which some nutrients become deficient, examination of the relationship between glycerol concentrations and acetate production by the cell, and the effect of the acetate level on the cell growth rate.
520 $a The work shows that a relatively high cell density was achieved, but the protein production was still relatively low. Nevertheless, the fermentation process developed in this work provides a foundation that others could use to develop a robust high productivity system using E. coli strain GI724.
590 $a School code: 0111.
650 4 $a Engineering, Chemical. $3 1018531
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0542
690 $a 0410
710 2 0 $a University of Massachusetts Lowell. $3 1017839
773 0 $t Masters Abstracts International $g 43-05.
790 1 0 $a Lawton, Carl, $e advisor
790 $a 0111
791 $a M.S.Eng.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1425526