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Consensus antigens for New World are...

Archer, Angela Marie.

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Consensus antigens for New World arenavirus immunization.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Consensus antigens for New World arenavirus immunization./
作者:	Archer, Angela Marie.
面頁冊數:	138 p.
附註:	Source: Dissertation Abstracts International, Volume: 64-08, Section: B, page: 3732.
Contained By:	Dissertation Abstracts International64-08B.
標題:	Health Sciences, Immunology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3102476
ISBN:	0496500465

Consensus antigens for New World arenavirus immunization.
Archer, Angela Marie.

Consensus antigens for New World arenavirus immunization. - 138 p.

Source: Dissertation Abstracts International, Volume: 64-08, Section: B, page: 3732.

Thesis (Ph.D.)--The University of Texas Health Science Center at San Antonio, 2003.

The Arenaviridae are enveloped, single-stranded, bisegmented RNA viruses found throughout the world. These rodent- and bat-borne viruses are divided into two major groups, the Old World and New World Complex, based primarily on antigenic typing. Of the 18 known New World arenaviruses, at least five (Junin, Machupo, Sabia, Guanarito, and Whitewater Arroyo) have been associated with hemorrhagic fever in humans.

ISBN: 0496500465Subjects--Topical Terms:

1017716
Health Sciences, Immunology.

Consensus antigens for New World arenavirus immunization.
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500 $a Source: Dissertation Abstracts International, Volume: 64-08, Section: B, page: 3732.
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502 $a Thesis (Ph.D.)--The University of Texas Health Science Center at San Antonio, 2003.
520 $a The Arenaviridae are enveloped, single-stranded, bisegmented RNA viruses found throughout the world. These rodent- and bat-borne viruses are divided into two major groups, the Old World and New World Complex, based primarily on antigenic typing. Of the 18 known New World arenaviruses, at least five (Junin, Machupo, Sabia, Guanarito, and Whitewater Arroyo) have been associated with hemorrhagic fever in humans.
520 $a RT-PCR protocols were developed to amplify and determine the sequence of the S segment of the New World arenaviruses for which information was unavailable: Allpahuayo, Amapari, Flexal, Guanarito, Latino, Machupo, Parana, Pirital, Tamiami, and Whitewater Arroyo. These sequences were then used to more accurately predict their evolutionary relationships and by analysis of amino acids encoded by these sequences, two regions were identified that are conserved and may have immunogenic potential.
520 $a Short peptides were synthesized to reflect a consensus of all the New World arenaviruses at the two domains identified above, and were used to immunize guinea pigs. One peptide, representing a domain within the GP2, elicited a humoral response, but was not reactive with whole virus in an ELISA. A second peptide, corresponding to a domain in the nucleocapsid protein, did not elicit a humoral response.
520 $a Previous work suggested that glycosylation of immunogens might play a role in inducing an anti-viral immune response, so a second approach was used to generate a cross-reactive immunogen with the appropriate processing. A Sindbis virus vector system was used to express the glycoprotein precursor of Guanarito virus in mammalian cells, for the preparation of a 43-amino acid peptide corresponding to the glycosylated GP2 domain. However, after purifying the protein, it was determined that the product had probably not been processed correctly. The glycoprotein precursor was probably not cleaved into subunits, GP1 and GP2, and the conformational hindrances that resulted probably blocked digestion and purification of the 43-amino acid peptide target. Other methods are needed to prepare the minimal antigenic motifs necessary for producing a cross-reactive New World arenavirus immunogen. As the number of arenaviruses isolated continues to grow, there is renewed urgency to develop vaccines against known species and soon-to-be discovered arenaviruses as well.
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