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Regulation of subgenomic mRNA transc...

Xu, Wei.

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Regulation of subgenomic mRNA transcription and translation in cucumber leaf spot virus.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Regulation of subgenomic mRNA transcription and translation in cucumber leaf spot virus./
作者:	Xu, Wei.
面頁冊數:	84 p.
附註:	Source: Dissertation Abstracts International, Volume: 72-09, Section: B, page: .
Contained By:	Dissertation Abstracts International72-09B.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NR75684
ISBN:	9780494756843

Regulation of subgenomic mRNA transcription and translation in cucumber leaf spot virus.
Xu, Wei.

Regulation of subgenomic mRNA transcription and translation in cucumber leaf spot virus. - 84 p.

Source: Dissertation Abstracts International, Volume: 72-09, Section: B, page: .

Thesis (Ph.D.)--York University (Canada), 2011.

The aureusvirus Cucumber leaf spot virus (CLSV) is a messenger-sense, single-stranded RNA plant virus that belongs to the family Tombusviridae. The &sim;4.4 kb long genome encodes five viral proteins. However, the three viral proteins encoded near the 3' end are translationally silent in the viral genome. Their expression requires the transcription of two smaller viral subgenomic (sg) mRNAs that template their translation. Since all viral proteins are essential for a successful CLSV infection of plant hosts, the transcription of sg mRNAs plays a critical role in this process. The purpose of my study is to understand the regulation of transcription and translation of the two sg mRNAs that are produced by CLSV during infections. Accordingly, I carried out in vivo and in vitro studies to characterize RNA structural elements involved in these two processes. My research identified different RNA elements (i.e. attenuation structures, promoters and spacer sequences) involved in sg mRNA transcription and analysis of the function of these elements indicated that CLSV synthesizes its sg mRNAs by a premature termination mechanism. Further analysis of the translational mechanism of sg mRNA1 indicated that it uses a mechanism that is independence of 5'cap- and a 3'poly(A)-tail to translate its coat protein. This unusual translational mechanism requires a long-range RNA-RNA interaction between the 5'untranslated region of sg mRNA1 and a translational enhancer in the 3'UTR of the same sg mRNA1. Overall, my findings provide important new details about the role of RNA elements in the processes of CLSV sg mRNA transcription and translation and extend our understanding of the regulation of these important processes during viral infections.

ISBN: 9780494756843Subjects--Topical Terms:

1017734
Biology, Microbiology.

Regulation of subgenomic mRNA transcription and translation in cucumber leaf spot virus.
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520 $a The aureusvirus Cucumber leaf spot virus (CLSV) is a messenger-sense, single-stranded RNA plant virus that belongs to the family Tombusviridae. The &sim;4.4 kb long genome encodes five viral proteins. However, the three viral proteins encoded near the 3' end are translationally silent in the viral genome. Their expression requires the transcription of two smaller viral subgenomic (sg) mRNAs that template their translation. Since all viral proteins are essential for a successful CLSV infection of plant hosts, the transcription of sg mRNAs plays a critical role in this process. The purpose of my study is to understand the regulation of transcription and translation of the two sg mRNAs that are produced by CLSV during infections. Accordingly, I carried out in vivo and in vitro studies to characterize RNA structural elements involved in these two processes. My research identified different RNA elements (i.e. attenuation structures, promoters and spacer sequences) involved in sg mRNA transcription and analysis of the function of these elements indicated that CLSV synthesizes its sg mRNAs by a premature termination mechanism. Further analysis of the translational mechanism of sg mRNA1 indicated that it uses a mechanism that is independence of 5'cap- and a 3'poly(A)-tail to translate its coat protein. This unusual translational mechanism requires a long-range RNA-RNA interaction between the 5'untranslated region of sg mRNA1 and a translational enhancer in the 3'UTR of the same sg mRNA1. Overall, my findings provide important new details about the role of RNA elements in the processes of CLSV sg mRNA transcription and translation and extend our understanding of the regulation of these important processes during viral infections.
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