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Characterization of the ARF guanine ...

Imboden-Davison, Lori A.

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Characterization of the ARF guanine nucleotide exchange factor MIN7 and the RabE1 GTPases of Arabidopsis.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Characterization of the ARF guanine nucleotide exchange factor MIN7 and the RabE1 GTPases of Arabidopsis./
作者:	Imboden-Davison, Lori A.
面頁冊數:	123 p.
附註:	Source: Dissertation Abstracts International, Volume: 72-08, Section: B, page: .
Contained By:	Dissertation Abstracts International72-08B.
標題:	Biology, Botany. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3454745
ISBN:	9781124646909

Characterization of the ARF guanine nucleotide exchange factor MIN7 and the RabE1 GTPases of Arabidopsis.
Imboden-Davison, Lori A.

Characterization of the ARF guanine nucleotide exchange factor MIN7 and the RabE1 GTPases of Arabidopsis. - 123 p.

Source: Dissertation Abstracts International, Volume: 72-08, Section: B, page: .

Thesis (Ph.D.)--Michigan State University, 2011.

Pseudomonas syringae pv tomato DC3000 is a Gram-negative bacterium that utilizes a type three secretion system to translocate effector proteins into plant cells to promote pathogenesis. There are &sim;30 effectors secreted by Pst DC3000 and the effectors HopM1 and AvrPto have both been implicated in targeting host vesicle trafficking systems. HopM1 has been shown to promote disease by targeting Hop M1 interactor 7 (MIN7). MIN7 contains the SEC7 domain, the catalytic domain of ADP-ribosylation factor (ARF) guanine nucleotide exchange factors (GEFs). ARF GEFs promote the exchange of GTP for GDP on ARF GTPases, but GEF activity has not been demonstrated for MIN7. In this research I show that the SEC7 domain of MIN7 is capable of promoting the exchange of GTP for GDP on an Arabidopsis ARF GTPase. In addition, I found that MIN7::DsRed was partially co-localized with at least five Arabidopsis ARFs.

ISBN: 9781124646909Subjects--Topical Terms:

1017825
Biology, Botany.

Characterization of the ARF guanine nucleotide exchange factor MIN7 and the RabE1 GTPases of Arabidopsis.
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245 1 0 $a Characterization of the ARF guanine nucleotide exchange factor MIN7 and the RabE1 GTPases of Arabidopsis.
300 $a 123 p.
500 $a Source: Dissertation Abstracts International, Volume: 72-08, Section: B, page: .
500 $a Adviser: Sheng Yang He.
502 $a Thesis (Ph.D.)--Michigan State University, 2011.
520 $a Pseudomonas syringae pv tomato DC3000 is a Gram-negative bacterium that utilizes a type three secretion system to translocate effector proteins into plant cells to promote pathogenesis. There are &sim;30 effectors secreted by Pst DC3000 and the effectors HopM1 and AvrPto have both been implicated in targeting host vesicle trafficking systems. HopM1 has been shown to promote disease by targeting Hop M1 interactor 7 (MIN7). MIN7 contains the SEC7 domain, the catalytic domain of ADP-ribosylation factor (ARF) guanine nucleotide exchange factors (GEFs). ARF GEFs promote the exchange of GTP for GDP on ARF GTPases, but GEF activity has not been demonstrated for MIN7. In this research I show that the SEC7 domain of MIN7 is capable of promoting the exchange of GTP for GDP on an Arabidopsis ARF GTPase. In addition, I found that MIN7::DsRed was partially co-localized with at least five Arabidopsis ARFs.
520 $a min7 plants are compromised in salicylic acid (SA)-dependent defense and display hypersensitive cell death to benzothiadiazole (BTH), a functional analogue of SA. Two Arabidopsis lines with T-DNA insertions in the ARF-like B1 (ARL-B1) gene are found to be hypersensitive to BTH. Unlike min7 plants, however, the ARL-B1 knockout plants are not compromised in BTH-induced defense. Thus, BTH hypersensitivity and deficient BTH-induced defense can be uncoupled.
520 $a AvrPto is an effector that compromises plant cell wall-based defense. AvrPto interacts in the yeast two-hybrid system with the RabE1 GTPases, a family of Rab GTPases predicted to be involved in polarized secretion in Arabidopsis. I found that AvrPto interacts with wild type RAB-E1d and RAB-E1d-Q74L (predicted to be GTP bound and active) but not with RAB-E1d-S29N (predicted to be GDP-bound and inactive). To better understand the function of RabE1 in the cell, I used the yeast two-hybrid screen to identify two Arabidopsis interactors of RabE1, REI1 and REI2. REI1 is annotated as a receptor-like kinase, and REI2 contains a SEC14-like domain.
590 $a School code: 0128.
650 4 $a Biology, Botany. $3 1017825
650 4 $a Biology, Cell. $3 1017686
650 4 $a Agriculture, Plant Pathology. $3 1028950
690 $a 0309
690 $a 0379
690 $a 0480
710 2 $a Michigan State University. $b Cell and Molecular Biology. $3 1684937
773 0 $t Dissertation Abstracts International $g 72-08B.
790 1 0 $a He, Sheng Yang, $e advisor
790 1 0 $a Keegstra, Kenneth $e committee member
790 1 0 $a Walton, Jonathan $e committee member
790 1 0 $a Thiem, Suzanne $e committee member
790 1 0 $a Allison, Richard $e committee member
790 $a 0128
791 $a Ph.D.
792 $a 2011
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