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Identification and characterization ...

Woo, Seung-Hyun.

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Identification and characterization of epithelial progenitor niches in skin.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Identification and characterization of epithelial progenitor niches in skin./
作者:	Woo, Seung-Hyun.
面頁冊數:	164 p.
附註:	Source: Dissertation Abstracts International, Volume: 72-07, Section: B, page: .
Contained By:	Dissertation Abstracts International72-07B.
標題:	Health Sciences, Pathology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3454033
ISBN:	9781124627267

Identification and characterization of epithelial progenitor niches in skin.
Woo, Seung-Hyun.

Identification and characterization of epithelial progenitor niches in skin. - 164 p.

Source: Dissertation Abstracts International, Volume: 72-07, Section: B, page: .

Thesis (Ph.D.)--Columbia University, 2011.

The skin contains a highly regionalized stem and progenitor cell niche system, where a particular epithelial progenitor population primarily displays unipotent or bipotent behavior by contributing to their local niche under homeostatic conditions. Some of these populations, however, can also contribute to lineages beyond their local niche in response to wounding or other traumas.

ISBN: 9781124627267Subjects--Topical Terms:

1017854
Health Sciences, Pathology.

Identification and characterization of epithelial progenitor niches in skin.
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245 1 0 $a Identification and characterization of epithelial progenitor niches in skin.
300 $a 164 p.
500 $a Source: Dissertation Abstracts International, Volume: 72-07, Section: B, page: .
500 $a Adviser: David M. Owens.
502 $a Thesis (Ph.D.)--Columbia University, 2011.
520 $a The skin contains a highly regionalized stem and progenitor cell niche system, where a particular epithelial progenitor population primarily displays unipotent or bipotent behavior by contributing to their local niche under homeostatic conditions. Some of these populations, however, can also contribute to lineages beyond their local niche in response to wounding or other traumas.
520 $a The goal of this thesis was to identify additional epithelial progenitor niches in the skin and characterize them. I focused on identifying and investigating the role of epithelial progenitors residing in two discrete compartments, the upper isthmus (UI) of the hair follicle and the touch dome (TD) in the epidermis, during skin development and homeostasis.
520 $a The UI niche was identified by immunofluorescence studies based on the expression profile of alpha6 integrinlow Sca-1- CD34- cells. The progenitor characteristics of UI keratinocytes were confirmed using skin reconstitution and in vitro colony formation assays. The transcriptional profiling of UI cells led to the identification of Tbc1d10c, one of the uniquely upregulated genes in UI cells. The comprehensive characterization of the UI niche by immunofluorescence led to the identification of a neural complex called palisade nerve endings (PN) present outside of the UI. Further investigation of PN using immunofluorescence and animal models revealed the presence of a glutamate transport to the HF via sensory nerve fibers. I elucidated that this glutamate influx was critical for the organization of Schwann cells in the developing HF. Although the relationship between UI keratinocytes and PN still needs to be investigated, the presence of PN in the UI niche implicates that there is more function ascribed to UI cells in addition to serving as epithelial progenitors.
520 $a The next epithelial progenitor niche I identified and characterized was the touch dome (TD) in the epidermis of hairy skin. The TD is a specialized niche, in which both epidermal Merkel cells and TD keratinocytes reside. The unexpected and exclusive presence of both Tbc1d10c and CD200 proteins in TD keratinocytes eventually led us to find that they were epidermal Merkel cell progenitors. Their progenitor characteristics were validated using skin reconstitution assay and in vivo lineage tracing by EdU incorporation. Our results collectively suggested that TD keratinocytes were bipotent progenitors that gave rise to both squamous and neuroendocrine epidermal lineages.
520 $a Lastly, I generated a UI-specific Cre transgenic mouse line driven by the Tbc1d10c promoter, which will be utilized for further characterization of UI keratinocytes, including in vivo lineage tracing. I also generated Tbc1d10c complete knockout mice, which I will investigate to determine whether Tbc1d10c plays a role during skin development and homeostasis.
590 $a School code: 0054.
650 4 $a Health Sciences, Pathology. $3 1017854
650 4 $a Health Sciences, Human Development. $3 1019218
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690 $a 0758
710 2 $a Columbia University. $b Pathobiology and Molecular Medicine. $3 1684919
773 0 $t Dissertation Abstracts International $g 72-07B.
790 1 0 $a Owens, David M., $e advisor
790 $a 0054
791 $a Ph.D.
792 $a 2011
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