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Neorickettsia spp.: Molecular Classi...

Gibson, Kathryn Elizabeth.

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Neorickettsia spp.: Molecular Classification of a Vector and Roles of Bacterial Surface Proteins in Pathogenesis.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Neorickettsia spp.: Molecular Classification of a Vector and Roles of Bacterial Surface Proteins in Pathogenesis./
作者:	Gibson, Kathryn Elizabeth.
面頁冊數:	209 p.
附註:	Source: Dissertation Abstracts International, Volume: 72-06, Section: B, page: .
Contained By:	Dissertation Abstracts International72-06B.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3451738
ISBN:	9781124578682

Neorickettsia spp.: Molecular Classification of a Vector and Roles of Bacterial Surface Proteins in Pathogenesis.
Gibson, Kathryn Elizabeth.

Neorickettsia spp.: Molecular Classification of a Vector and Roles of Bacterial Surface Proteins in Pathogenesis. - 209 p.

Source: Dissertation Abstracts International, Volume: 72-06, Section: B, page: .

Thesis (Ph.D.)--The Ohio State University, 2011.

Neorickettsia spp. are Gram-negative, obligate intracellular bacteria of the family Anaplasmataceae. Given their prevalence throughout the world, their propensity to cause human disease and deadly animal diseases, and the continuing discovery of new Neorickettsia spp., they are significant pathogens requiring better comprehension. The overall objective of this dissertation is to determine the host-bacterium relationships of Neorickettsia. Chapter 1 details background on Neorickettsia spp., with emphasis on Neorickettsia risticii and Neorickettsia sennetsu. The objective of Chapter 2 was to demonstrate the lineage of all N. risticii-infected trematode life stages. The study established the molecular identification of the N. risticii adult trematode host and its immature life stages, demonstrating as hypothesized that all life stages harboring N. risticii belong to the same clade. The objective of Chapter 3 was to determine the major surface proteins of N. sennetsu involved in host-pathogen interaction and to determine the roles of the major surface proteins. Four proteins: the 51-kDa antigen (P51), Neorickettsia surface proteins 2 (Nsp2) and 3 (Nsp3), and heat-shock protein 60 (GroEL), were found to have the highest surface expression. It was hypothesized that the two major beta-barrel proteins, P51 and Nsp3 function as porins. The outer membrane fraction of N. sennetsu, as well as native P51 and Nsp3 were incorporated into proteoliposomes and tested by porin-swelling assays, and it was confirmed that P51 is a large porin. The objective of Chapter 4 was to determine levels of variation within predicted surface-exposed proteins of N. risticii , with the hypothesis being that geographic and temporal variation would occur among strains of N. risticii. Variation in P51 demonstrated geographic separation of N. risticii strains. Nsp2, Nsp3, and strain-specific antigen 3 (Ssa3) demonstrated temporal variation. Variety within the beta-barrel proteins P51, Nsp2, and Nsp3 occurred mainly within regions predicted as external loops. Ssa3 variation mainly occurred in an N-terminal localized repeat region and consisted of changes in the number of 52-aa repeats. The objective of Chapter 5 was to determine causes of cytokine and chemokine induction in N. sennetsu infection, thus potential reasons for disease symptoms. The studies in this chapter first validated the mouse model of Sennetsu neorickettsiosis in immunocompetent BALB/c mice and then demonstrated cytokine and chemokine production by quantitative reverse-transcriptase polymerase chain reaction within spleens of infected mice during disease. Cytokine and chemokine production in the whole mouse was replicated in vitro within splenocyte and bone marrow-derived macrophage (BMDM) cultures, with significant induction of IL-1beta, CXCL2, and IL-12A (p35) mRNAs occurring within whole bacteria and the Sarkosyl-purified bacterial outer membrane fraction of N. sennetsu. It was hypothesized that P51 is a major cause of cytokine induction, is recognized by Toll-like receptor 2, and that cytokine/chemokine induction is MyD88 dependent. Preliminary studies showed that cytokine/chemokine levels were reduced in MyD88-knockout mouse BMDMs and TLR2-knockout mouse splenocytes incubated with whole bacteria and in TLR2-knockout mouse BMDMs incubated with whole bacteria and bacterial outer membrane fraction. In conclusion, these studies demonstrated important host-pathogen relationships during Neorickettsial infection and disease useful for further understanding of these bacteria.

ISBN: 9781124578682Subjects--Topical Terms:

1017734
Biology, Microbiology.

Neorickettsia spp.: Molecular Classification of a Vector and Roles of Bacterial Surface Proteins in Pathogenesis.
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