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A PCR based HIV-1 test for point-of-...

Jangam, Sujit Rao.

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A PCR based HIV-1 test for point-of-care application.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	A PCR based HIV-1 test for point-of-care application./
作者:	Jangam, Sujit Rao.
面頁冊數:	235 p.
附註:	Source: Dissertation Abstracts International, Volume: 71-10, Section: B, page: 6262.
Contained By:	Dissertation Abstracts International71-10B.
標題:	Engineering, Biomedical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3419048
ISBN:	9781124184197

A PCR based HIV-1 test for point-of-care application.
Jangam, Sujit Rao.

A PCR based HIV-1 test for point-of-care application. - 235 p.

Source: Dissertation Abstracts International, Volume: 71-10, Section: B, page: 6262.

Thesis (Ph.D.)--Northwestern University, 2010.

HIV-1 proviral DNA detection by PCR is the gold standard test for early diagnosis in infants. In resource limited settings, dried blood samples are transported from the point-of-care (POC) to central laboratories for testing because the test requires complex equipment and skilled personnel. Unfortunately, transport problems frequently prevent result delivery to the physician/patient at the POC leading to high loss to follow up. We have developed a very simple, rapid and accurate real-time PCR based test for POC testing. The method employs a novel DNA extraction technology that uses single-use disposables to extract genomic DNA from blood in less than 5 minutes. The DNA is then analyzed by a multiplexed real-time PCR assay that has an exogenous internal control that reports on PCR inhibition in addition to the primers and probes for HIV-1 detection. Our method demonstrated 97% clinical sensitivity and 100% specificity when tested with infant blood specimens. The method is capable of detecting 5 HIV-1 copies in 100microl of blood. The method also allows for storage of blood samples for at least one month after blood collection. In order to make the test field-deployable, single-use devices have been developed for blood collection and DNA separation. Furthermore, a single-use assay card has been developed for performing the real-time PCR reaction with extracted genomic DNA. The assay card integrates DNA extraction, PCR reagent storage and PCR amplification. A prototype field-deployable portable analyzer has also been developed. The analyzer is battery-operated and is capable of assembling the reaction, fluorescence detection and thermal cycling. A proof-of-concept and design feasibility for each of the components of the test has been demonstrated.

ISBN: 9781124184197Subjects--Topical Terms:

1017684
Engineering, Biomedical.

A PCR based HIV-1 test for point-of-care application.
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