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Simultaneous saccharification and fe...

Pessani, Naveen K.

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Simultaneous saccharification and fermentation of switchgrass by thermotolerant Kluyveromyces marxianus IMB3 : Effect of enzyme loading, temperature and operating mode.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Simultaneous saccharification and fermentation of switchgrass by thermotolerant Kluyveromyces marxianus IMB3 : Effect of enzyme loading, temperature and operating mode./
作者:	Pessani, Naveen K.
面頁冊數:	113 p.
附註:	Source: Masters Abstracts International, Volume: 49-06, page: .
Contained By:	Masters Abstracts International49-06.
標題:	Alternative Energy. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1495092
ISBN:	9781124714097

Simultaneous saccharification and fermentation of switchgrass by thermotolerant Kluyveromyces marxianus IMB3 : Effect of enzyme loading, temperature and operating mode.
Pessani, Naveen K.

Simultaneous saccharification and fermentation of switchgrass by thermotolerant Kluyveromyces marxianus IMB3 : Effect of enzyme loading, temperature and operating mode. - 113 p.

Source: Masters Abstracts International, Volume: 49-06, page: .

Thesis (M.S.)--Oklahoma State University, 2011.

Scope and Method of Study: The objectives of this research were to determine the optimum enzyme (Accellerase 1500, Genencor International, NY, USA) loading, temperature and operating modes (batch and fed-batch) on ethanol production by simultaneous saccharification and fermentation (SSF) of pretreated Kanlow switchgrass using thermotolerant Kluyveromyces marxianus IMB3. Hydrothermolysis pretreatment of switchgrass was used. Also, 8% solids (dry basis, db) loading was used for the effect of enzyme loadings and temperatures tests. Various loadings of Accellerase 1500 (0.3, 0.5 and 0.7 mL g -1 glucan) were used in SSF at 45°C (activity of enzyme was 82.2 FPU mL-1). Then, SSFs were performed at 37, 41 and 45°C with the optimum enzyme loading. Finally, four different batch and fed-batch strategies were evaluated at the optimum enzyme loading and temperature with a total solid loading of 12% (db).

ISBN: 9781124714097Subjects--Topical Terms:

1035473
Alternative Energy.

Simultaneous saccharification and fermentation of switchgrass by thermotolerant Kluyveromyces marxianus IMB3 : Effect of enzyme loading, temperature and operating mode.
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245 1 0 $a Simultaneous saccharification and fermentation of switchgrass by thermotolerant Kluyveromyces marxianus IMB3 : Effect of enzyme loading, temperature and operating mode.
300 $a 113 p.
500 $a Source: Masters Abstracts International, Volume: 49-06, page: .
500 $a Adviser: Hasan K. Atiyeh.
502 $a Thesis (M.S.)--Oklahoma State University, 2011.
520 $a Scope and Method of Study: The objectives of this research were to determine the optimum enzyme (Accellerase 1500, Genencor International, NY, USA) loading, temperature and operating modes (batch and fed-batch) on ethanol production by simultaneous saccharification and fermentation (SSF) of pretreated Kanlow switchgrass using thermotolerant Kluyveromyces marxianus IMB3. Hydrothermolysis pretreatment of switchgrass was used. Also, 8% solids (dry basis, db) loading was used for the effect of enzyme loadings and temperatures tests. Various loadings of Accellerase 1500 (0.3, 0.5 and 0.7 mL g -1 glucan) were used in SSF at 45°C (activity of enzyme was 82.2 FPU mL-1). Then, SSFs were performed at 37, 41 and 45°C with the optimum enzyme loading. Finally, four different batch and fed-batch strategies were evaluated at the optimum enzyme loading and temperature with a total solid loading of 12% (db).
520 $a Findings and Conclusions: The optimum enzyme loading was 0.7 mL g -1 glucan in SSF with 8% solids at 45°C, which resulted in the highest ethanol concentration of 22.3 g L-1. This was equivalent to 85% maximum theoretical yield (MTY). For the effect of temperature experiment, the highest ethanol concentration of 23.0 g L-1 (86.3% MTY) was obtained in SSF with 8% solids at 45°C compared to about 20 g L -1 ethanol (77% MTY) obtained at both 37°C and 41°C. In addition, the results showed that about 32 g L-1 ethanol (81% MTY) was produced with a total solid loading of 12% (db) in all batch and fed-batch SSFs strategies. No significant differences in the amount of ethanol produced in batch or fed-batch operating modes were observed in the range of solids and enzyme loadings used (p > 0.05). Moreover, the results also showed that with fed-batch mode and 12% solids (db), the enzyme loading can be decreased by 33% of the optimum loading for batch process.
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