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Fermentation of xylose and xylans by...

Mueller, Michael.

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Fermentation of xylose and xylans by Kluyveromyces marxianus IMB strains.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Fermentation of xylose and xylans by Kluyveromyces marxianus IMB strains./
作者:	Mueller, Michael.
面頁冊數:	165 p.
附註:	Source: Masters Abstracts International, Volume: 47-06, page: 3651.
Contained By:	Masters Abstracts International47-06.
標題:	Engineering, Agricultural. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1465042
ISBN:	9781109174151

Fermentation of xylose and xylans by Kluyveromyces marxianus IMB strains.
Mueller, Michael.

Fermentation of xylose and xylans by Kluyveromyces marxianus IMB strains. - 165 p.

Source: Masters Abstracts International, Volume: 47-06, page: 3651.

Thesis (M.S.)--Oklahoma State University, 2009.

Scope and Method of Study. The first objective was to analyze five Kluyveromyces marxianus IMB strains on their ability to ferment xylose as sole carbon source at thermophilic conditions. Based on the findings a Simultaneous saccharification and fermentation (SSF) with IMB2, which performed the best in the first step, and Multifect Xylanase was performed at thermophilic conditions with xylan as substrate. Different enzyme concentrations were compared. To enhance ethanol production on xylose a co-culture fermentation with IMB2 and Pichia stipitis was conducted. Since P. stipitis has an optimum temperature at 30°C, the fermentation was performed at 30°C.

ISBN: 9781109174151Subjects--Topical Terms:

1019504
Engineering, Agricultural.

Fermentation of xylose and xylans by Kluyveromyces marxianus IMB strains.
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245 1 0 $a Fermentation of xylose and xylans by Kluyveromyces marxianus IMB strains.
300 $a 165 p.
500 $a Source: Masters Abstracts International, Volume: 47-06, page: 3651.
500 $a Adviser: Mark R. Wilkins.
502 $a Thesis (M.S.)--Oklahoma State University, 2009.
520 $a Scope and Method of Study. The first objective was to analyze five Kluyveromyces marxianus IMB strains on their ability to ferment xylose as sole carbon source at thermophilic conditions. Based on the findings a Simultaneous saccharification and fermentation (SSF) with IMB2, which performed the best in the first step, and Multifect Xylanase was performed at thermophilic conditions with xylan as substrate. Different enzyme concentrations were compared. To enhance ethanol production on xylose a co-culture fermentation with IMB2 and Pichia stipitis was conducted. Since P. stipitis has an optimum temperature at 30°C, the fermentation was performed at 30°C.
520 $a Findings and Conclusions. The screening of the IMB strains showed that IMB2 performed the best at 40°C on xylose fermentation with an ethanol yield of 0.07g/g and a xylitol yield of 0.42g/g. IMB2 showed in the SSF experiment the best ethanol production at 40°C with 18.6% of the theoretical maximum yield with no significant difference in enzyme concentration. Xylitol production was best at 40°C with 15.8% of the theoretical maximum yield and 2mul/ml enzyme concentration. The monoculture of IMB2 showed poor results in ethanol production at the co-culture conditions. The co-culture showed better results in ethanol production which was derived by P. stipitis. P. stipitis as monoculture had the highest conversion efficiency with 84%. An interesting finding was the high xylitol production by IMB2 as monoculture. The conversion efficiency was 65% and is comparable to other in the literature mentioned yeast strains. It can be concluded that the K. marxianus IMB strains are not suitable for ethanol production at thermophilic conditions with xylose as sole carbon source. K. marxianus was found as a potential strains for xylitol production.
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790 1 0 $a Mort, Andrew $e committee member
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792 $a 2009
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