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Isolation and characterization of hi...

Richard, Gabrielle.

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Isolation and characterization of high affinity VHH antibody fragments against alpha-cobratoxin.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Isolation and characterization of high affinity VHH antibody fragments against alpha-cobratoxin./
作者:	Richard, Gabrielle.
面頁冊數:	117 p.
附註:	Source: Masters Abstracts International, Volume: 48-05, page: 2946.
Contained By:	Masters Abstracts International48-05.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MR58412
ISBN:	9780494584125

Isolation and characterization of high affinity VHH antibody fragments against alpha-cobratoxin.
Richard, Gabrielle.

Isolation and characterization of high affinity VHH antibody fragments against alpha-cobratoxin. - 117 p.

Source: Masters Abstracts International, Volume: 48-05, page: 2946.

Thesis (M.Sc.)--University of Guelph (Canada), 2010.

Camelid VHHs may provide better treatment for snake envenomation than conventional antivenom antibodies because of their smaller size (&sim;16 kDa), better tissue permeability and lower immunogenicity. In this thesis, a phage-displayed VHH library with 4.2 x 109 functional clones was constructed from a Ilama hyperimmunized with crude Thai cobra ( Naja kaouthia) venom. After three rounds of panning against acobratoxin (alpha-Cbtx), a potent neurotoxin from the Thai cobra venom, 26 unique clones were found using monoclonal phage ELISA and confirmed by DNA sequencing. Surface plasmon resonance (SPR) analyses showed that the four selected anti-alpha-Cbtx VHH clones had dissociation constants (KD) in the low nanomolar range (0.4-25 nM)and that these four VHHs bound to the same or overlapping epitopes on alpha-Cbtx. An in vitro muscle twitch assay showed that VHH C2 (KD = 0.4nM) effectively neutralized the paralytic effects of alpha-Cbtx at neuromuscular junctions.

ISBN: 9780494584125Subjects--Topical Terms:

1017719
Biology, Molecular.

Isolation and characterization of high affinity VHH antibody fragments against alpha-cobratoxin.
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