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Effects of dietary aflatoxin on the ...

Rustemeyer, Sheila M.

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Effects of dietary aflatoxin on the performance and hepatic gene expression of growing barrows.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Effects of dietary aflatoxin on the performance and hepatic gene expression of growing barrows./
作者:	Rustemeyer, Sheila M.
面頁冊數:	101 p.
附註:	Source: Masters Abstracts International, Volume: 49-02, page: 0940.
Contained By:	Masters Abstracts International49-02.
標題:	Agriculture, Animal Culture and Nutrition. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1482392
ISBN:	9781124259871

Effects of dietary aflatoxin on the performance and hepatic gene expression of growing barrows.
Rustemeyer, Sheila M.

Effects of dietary aflatoxin on the performance and hepatic gene expression of growing barrows. - 101 p.

Source: Masters Abstracts International, Volume: 49-02, page: 0940.

Thesis (M.S.)--University of Wyoming, 2010.

Aflatoxins, especially aflatoxin B1 (AFB1), can be high in dried distillers grains with solubles (DDGS) when concentrated during the ethanol production process. Increased use of DDGS in swine diets could potentially lead to an increased incidence of aflatoxicosis, a disease associated with decreased feed intake, reduced BW gain, and impaired liver function. The objectives of this study were to determine the effects of AFB1 on (1) the health, performance, and serum profile and (2) the hepatic gene expression of growing barrows. Duroc x Yorkshire crossbred barrows (n = 90; age = 35 +/- 5 d; initial BW = 14.2 +/- 3.0 kg) were randomly assigned in a 3 x 3 factorial design to receive 0 (CON), 250 ppb AFB1 (LOW), or 500 ppb AFB1 (HIGH) for 7, 28, or 70 d. Feed intake was measured daily, and pigs were weighed and blood samples collected weekly. Serum was analyzed for concentrations of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (BILI), and blood urea nitrogen (BUN). Average feed intake was lower (P < 0.01) in HIGH and LOW barrows than in CON barrows in wk 8 and 9, and was lower (P < 0.05) in HIGH barrows only in wk 10. Lower ADG (P < 0.05) was observed in HIGH barrows than in CON barrows in wk 8 and 10; no differences in ADG were noted between CON and LOW barrows. There was no effect of AFB1 treatment on ALT or BILI concentrations. However, both AST and BUN were affected (P < 0.05) by AFB1 treatment. Concentrations of AST were greater in both HIGH ( P = 0.067) and LOW (P = 0.073) barrows than CON barrows, while BUN concentrations were lower in both HIGH (P = 0.073) and LOW (P = 0.012) barrows.

ISBN: 9781124259871Subjects--Topical Terms:

1017857
Agriculture, Animal Culture and Nutrition.

Effects of dietary aflatoxin on the performance and hepatic gene expression of growing barrows.
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245 1 0 $a Effects of dietary aflatoxin on the performance and hepatic gene expression of growing barrows.
300 $a 101 p.
500 $a Source: Masters Abstracts International, Volume: 49-02, page: 0940.
500 $a Adviser: Kristi M. Cammack.
502 $a Thesis (M.S.)--University of Wyoming, 2010.
520 $a Aflatoxins, especially aflatoxin B1 (AFB1), can be high in dried distillers grains with solubles (DDGS) when concentrated during the ethanol production process. Increased use of DDGS in swine diets could potentially lead to an increased incidence of aflatoxicosis, a disease associated with decreased feed intake, reduced BW gain, and impaired liver function. The objectives of this study were to determine the effects of AFB1 on (1) the health, performance, and serum profile and (2) the hepatic gene expression of growing barrows. Duroc x Yorkshire crossbred barrows (n = 90; age = 35 +/- 5 d; initial BW = 14.2 +/- 3.0 kg) were randomly assigned in a 3 x 3 factorial design to receive 0 (CON), 250 ppb AFB1 (LOW), or 500 ppb AFB1 (HIGH) for 7, 28, or 70 d. Feed intake was measured daily, and pigs were weighed and blood samples collected weekly. Serum was analyzed for concentrations of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (BILI), and blood urea nitrogen (BUN). Average feed intake was lower (P < 0.01) in HIGH and LOW barrows than in CON barrows in wk 8 and 9, and was lower (P < 0.05) in HIGH barrows only in wk 10. Lower ADG (P < 0.05) was observed in HIGH barrows than in CON barrows in wk 8 and 10; no differences in ADG were noted between CON and LOW barrows. There was no effect of AFB1 treatment on ALT or BILI concentrations. However, both AST and BUN were affected (P < 0.05) by AFB1 treatment. Concentrations of AST were greater in both HIGH ( P = 0.067) and LOW (P = 0.073) barrows than CON barrows, while BUN concentrations were lower in both HIGH (P = 0.073) and LOW (P = 0.012) barrows.
520 $a RNA-Seq was performed on liver samples from the d 70 groups. Forty-three functional groups were identified using DAVID, and genes within the apoptosis regulation functional group were selected for (1) validation of d 70 gene expression differences using real-time RT-PCR (n = 4 genes), and (2) investigation of d 7 expression to identify early response to aflatoxins (n = 15 genes). Expression levels of the four apoptosis genes selected for validation, CDKN1A, ZMAT3, KNG1, and PIM1, were confirmed with real-time RT-PCR. Of the 15 genes tested in the d 7 liver samples, 4 were differentially expressed: CDKN1A, ZMAT3, YWHAZ, and AEN. Results from this study demonstrate that administration of an AFB1-contaminated diet to growing barrows reduces performance, compromises health, and alters hepatic gene expression.
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