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Label-free comparative proteomic stu...

Fon, Mario G.

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Label-free comparative proteomic study of citrus fruit maturation.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Label-free comparative proteomic study of citrus fruit maturation./
Author:	Fon, Mario G.
Description:	52 p.
Notes:	Source: Masters Abstracts International, Volume: 48-02, page: 0862.
Contained By:	Masters Abstracts International48-02.
Subject:	Agriculture, Horticulture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1470282
ISBN:	9781109487473

Label-free comparative proteomic study of citrus fruit maturation.
Fon, Mario G.

Label-free comparative proteomic study of citrus fruit maturation. - 52 p.

Source: Masters Abstracts International, Volume: 48-02, page: 0862.

Thesis (M.S.)--University of California, Davis, 2009.

Fruit ripening and maturation are important not only for the plant, but for organisms that consume them. These processes are characterized by biochemical and physiological changes, with the fruit becoming more palatable. Fruits have been classically defined as being climacteric or non-climacteric, depending on the presence of a peak in respiration and ethylene production during ripening. Climacteric fruit ripening has been studied extensively, leading to increased knowledge of the regulation processes behind it. Non-climacteric fruits such as Citrus, however, have not yet been studied to this extent, and the knowledge is lacking in comparison. Proteomics is a powerful set of analytical techniques used to study the protein complement in a cell, tissue or organ that can lead to a better understanding of cellular processes. One advantage is that analysis of the proteins can give peptide sequences that can then be used to search for protein identities and functions, leading to identification of pathways that are regulated during physiological changes such as those occurring during fruit development. We used label-free proteomic methods to study the metabolic changes of two stages of Citrus fruit growth, 35mm fruit vs 55mm fruit and 55mm fruit vs 75 mm fruit. Our results showed that in the first comparison (35mm vs 55mm fruit), there was more regulation on proteins related to secondary metabolism (17%), followed by amino acid metabolism (14%), cell wall (11%), Major CHO (8%), redox (8%), among others. In the second comparison (55mm vs 75mm fruit), the metabolic assignment of proteins suggested shifts in metabolism, with glycolysis (16%) being the most represented, followed by cell wall (15%), secondary metabolism (13%), amino acid (10%), redox (10%), major CHO (9%), TCA cycle (9%), minor CHO (7%), photosynthesis (5%).

ISBN: 9781109487473Subjects--Topical Terms:

1017832
Agriculture, Horticulture.

Label-free comparative proteomic study of citrus fruit maturation.
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100 1 $a Fon, Mario G. $3 1677022
245 1 0 $a Label-free comparative proteomic study of citrus fruit maturation.
300 $a 52 p.
500 $a Source: Masters Abstracts International, Volume: 48-02, page: 0862.
500 $a Adviser: Eduardo Blumwald.
502 $a Thesis (M.S.)--University of California, Davis, 2009.
520 $a Fruit ripening and maturation are important not only for the plant, but for organisms that consume them. These processes are characterized by biochemical and physiological changes, with the fruit becoming more palatable. Fruits have been classically defined as being climacteric or non-climacteric, depending on the presence of a peak in respiration and ethylene production during ripening. Climacteric fruit ripening has been studied extensively, leading to increased knowledge of the regulation processes behind it. Non-climacteric fruits such as Citrus, however, have not yet been studied to this extent, and the knowledge is lacking in comparison. Proteomics is a powerful set of analytical techniques used to study the protein complement in a cell, tissue or organ that can lead to a better understanding of cellular processes. One advantage is that analysis of the proteins can give peptide sequences that can then be used to search for protein identities and functions, leading to identification of pathways that are regulated during physiological changes such as those occurring during fruit development. We used label-free proteomic methods to study the metabolic changes of two stages of Citrus fruit growth, 35mm fruit vs 55mm fruit and 55mm fruit vs 75 mm fruit. Our results showed that in the first comparison (35mm vs 55mm fruit), there was more regulation on proteins related to secondary metabolism (17%), followed by amino acid metabolism (14%), cell wall (11%), Major CHO (8%), redox (8%), among others. In the second comparison (55mm vs 75mm fruit), the metabolic assignment of proteins suggested shifts in metabolism, with glycolysis (16%) being the most represented, followed by cell wall (15%), secondary metabolism (13%), amino acid (10%), redox (10%), major CHO (9%), TCA cycle (9%), minor CHO (7%), photosynthesis (5%).
590 $a School code: 0029.
650 4 $a Agriculture, Horticulture. $3 1017832
650 4 $a Biology, Plant Physiology. $3 1017865
690 $a 0471
690 $a 0817
710 2 $a University of California, Davis. $b Horticulture and Agronomy. $3 1676792
773 0 $t Masters Abstracts International $g 48-02.
790 1 0 $a Blumwald, Eduardo, $e advisor
790 1 0 $a Blumwald, Eduardo $e committee member
790 1 0 $a Labavitch, John M. $e committee member
790 1 0 $a Negre-Zakharov, Florence $e committee member
790 $a 0029
791 $a M.S.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1470282