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Developing resistance to whitefly in...

Perera, Dinum.

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Developing resistance to whitefly in poinsettia (Euphorbia pulcherrima) using Agrobacterium-mediated transformation.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Developing resistance to whitefly in poinsettia (Euphorbia pulcherrima) using Agrobacterium-mediated transformation./
Author:	Perera, Dinum.
Description:	79 p.
Notes:	Source: Masters Abstracts International, Volume: 47-06, page: 3355.
Contained By:	Masters Abstracts International47-06.
Subject:	Agriculture, Horticulture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1467211
ISBN:	9781109272383

Developing resistance to whitefly in poinsettia (Euphorbia pulcherrima) using Agrobacterium-mediated transformation.
Perera, Dinum.

Developing resistance to whitefly in poinsettia (Euphorbia pulcherrima) using Agrobacterium-mediated transformation. - 79 p.

Source: Masters Abstracts International, Volume: 47-06, page: 3355.

Thesis (M.S.)--Mississippi State University, 2009.

The broad objective of this research was to develop transgenic poinsettia that express tryptophan decarboxylase (TDC) capable of protecting poinsettia against whitefly. An effective and efficient in vitro micro propagation and proliferation technique of poinsettia 'Prestige Red' was successfully developed in this study and this protocol can be used for potential development of transgenic poinsettia. Poinsettia 'Prestige Red' was successfully infected by Agrobacterium rhizogenes producing hairy roots at the site of infection. Investigations of more effective PGR concentrations are necessary in order to develop transgenic poinsettia through hairy roots. Stem disks of poinsettia 'Eckespoint Pollys Pink' developed into somatic embryos when they were transformed by A. tumefaciens harboring TDC. A. tumefaciens-mediated transformation of poinsettia through somatic embryogenesis is cultivar dependent. Additional research into more effective PGR combinations, antibiotic concentrations and antinecrosis chemicals is required in order to develop transgenic poinsettia harboring TDC through somatic embryogenesis using A. tumefaciens.

ISBN: 9781109272383Subjects--Topical Terms:

1017832
Agriculture, Horticulture.

Developing resistance to whitefly in poinsettia (Euphorbia pulcherrima) using Agrobacterium-mediated transformation.
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035 $a (UMI)AAI1467211
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100 1 $a Perera, Dinum. $3 1676961
245 1 0 $a Developing resistance to whitefly in poinsettia (Euphorbia pulcherrima) using Agrobacterium-mediated transformation.
300 $a 79 p.
500 $a Source: Masters Abstracts International, Volume: 47-06, page: 3355.
500 $a Adviser: Brian W. Trader.
502 $a Thesis (M.S.)--Mississippi State University, 2009.
520 $a The broad objective of this research was to develop transgenic poinsettia that express tryptophan decarboxylase (TDC) capable of protecting poinsettia against whitefly. An effective and efficient in vitro micro propagation and proliferation technique of poinsettia 'Prestige Red' was successfully developed in this study and this protocol can be used for potential development of transgenic poinsettia. Poinsettia 'Prestige Red' was successfully infected by Agrobacterium rhizogenes producing hairy roots at the site of infection. Investigations of more effective PGR concentrations are necessary in order to develop transgenic poinsettia through hairy roots. Stem disks of poinsettia 'Eckespoint Pollys Pink' developed into somatic embryos when they were transformed by A. tumefaciens harboring TDC. A. tumefaciens-mediated transformation of poinsettia through somatic embryogenesis is cultivar dependent. Additional research into more effective PGR combinations, antibiotic concentrations and antinecrosis chemicals is required in order to develop transgenic poinsettia harboring TDC through somatic embryogenesis using A. tumefaciens.
590 $a School code: 0132.
650 4 $a Agriculture, Horticulture. $3 1017832
690 $a 0471
710 2 $a Mississippi State University. $b Biochemistry and Molecular Biology. $3 1676962
773 0 $t Masters Abstracts International $g 47-06.
790 1 0 $a Trader, Brian W., $e advisor
790 1 0 $a Harkess, Richard L. $e committee member
790 1 0 $a Peterson, Daniel $e committee member
790 1 0 $a Caprio, Michael A. $e committee member
790 $a 0132
791 $a M.S.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1467211