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Responses of Arabidopsis to high lev...

Visscher, Anne Marieke.

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Responses of Arabidopsis to high levels of magnesium sulfate and of wheat to a spaceflight environment; consequences for (extra)terrestrial plant growth.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Responses of Arabidopsis to high levels of magnesium sulfate and of wheat to a spaceflight environment; consequences for (extra)terrestrial plant growth./
作者:	Visscher, Anne Marieke.
面頁冊數:	164 p.
附註:	Source: Dissertation Abstracts International, Volume: 71-11, Section: B, page: 6504.
Contained By:	Dissertation Abstracts International71-11B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3429139
ISBN:	9781124294698

Responses of Arabidopsis to high levels of magnesium sulfate and of wheat to a spaceflight environment; consequences for (extra)terrestrial plant growth.
Visscher, Anne Marieke.

Responses of Arabidopsis to high levels of magnesium sulfate and of wheat to a spaceflight environment; consequences for (extra)terrestrial plant growth. - 164 p.

Source: Dissertation Abstracts International, Volume: 71-11, Section: B, page: 6504.

Thesis (Ph.D.)--University of Florida, 2009.

The ability to utilize in situ resources is crucial for the success of extended manned space exploration of other planetary surfaces such as the Moon or Mars. Martian regolith containing potentially phytotoxic levels of elements is a potential medium for plant growth in bioregenerative life support systems. Studies of surface materials on Mars have detected a variety of hydrated sulfate minerals, including highly soluble magnesium sulfate minerals. Localized weight percentages of magnesium sulfate can reach 10% in the regolith. Levels of magnesium and sulfate ions toxic to crop plants have been described for soil types on Earth, such as serpentine and acid sulfate soils. We tested whether Arabidopsis knockout lines of genes encoding plasma membrane localized ion transporters in peripheral root cells can thrive in soils containing hyper elevated levels of magnesium sulfate. The selected mrs2-10 and sel1-10 mutant backgrounds do not mitigate the constraining impacts of high magnesium sulfate concentrations on wildtype plants. Based on these findings, a microarray experiment was done to characterize the early gene expression responses of col-0 Arabidopsis roots to elevated concentrations of magnesium sulfate. The cax1-1 mutant line, which has been shown to exhibit increased tolerance for high levels of magnesium, was also included in the experiment. The results for col-0 point to a reduction in root growth and vacuolar storage of magnesium and sulfate upon high magnesium sulfate treatment. Although many transporters or their potential regulators were differentially expressed, more research is needed to discover whether any of those are involved in magnesium sulfate transport. The down-regulated expression of cax1-1 is a natural response to high magnesium sulfate in Arabidopsis, and can explain the fact that only three transcripts were differentially expressed between cax1-1 and col-0. Furthermore, we tested whether changes in gene expression patterns can be detected in wheat plants that are several generations removed from growth in space, compared to control plants with no spaceflight exposure in their lineage. We found that none of the wheat genes represented by 10,263 probes on custom-made microarrays showed a statistically significant difference in expression.

ISBN: 9781124294698Subjects--Topical Terms:

1017719
Biology, Molecular.

Responses of Arabidopsis to high levels of magnesium sulfate and of wheat to a spaceflight environment; consequences for (extra)terrestrial plant growth.
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