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In vitro ecology of Calopogon tubero...

Kauth, Philip.

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In vitro ecology of Calopogon tuberosus var. tuberosus: A new concept in species conservation.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	In vitro ecology of Calopogon tuberosus var. tuberosus: A new concept in species conservation./
作者:	Kauth, Philip.
面頁冊數:	215 p.
附註:	Source: Dissertation Abstracts International, Volume: 71-03, Section: B, page: 1412.
Contained By:	Dissertation Abstracts International71-03B.
標題:	Biology, Botany. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3400272
ISBN:	9781109670929

In vitro ecology of Calopogon tuberosus var. tuberosus: A new concept in species conservation.
Kauth, Philip.

In vitro ecology of Calopogon tuberosus var. tuberosus: A new concept in species conservation. - 215 p.

Source: Dissertation Abstracts International, Volume: 71-03, Section: B, page: 1412.

Thesis (Ph.D.)--University of Florida, 2009.

The importance of ecotypic differentiation has recently been highlighted for plant conservation purposes, but use of local plant material for orchid restoration has been largely ignored. Using local plant material is necessary to maintain ecosystem and plant population stability, but little is known regarding the degree of ecotypic differentiation necessary to maintain stability. Ecotypic differentiation among geographically distant populations of the orchid, Calopogon tuberosus, was examined using in vitro ecology methods. Comparative effects of photoperiod, germination media, temperature, and chilling on asymbiotic in vitro seed germination and seedling development of C. tuberosus populations were examined. Seedling biomass allocation and corm dormancy were also examined. Regardless of germination treatment, Michigan seeds germinated and developed rapidly, while south Florida seeds germinated and developed slowly. This was likely due to the faster onset of winter conditions following seed dispersal in more northern environments. Photoperiod did not significantly influence seed germination and development. Different germination media significantly influenced germination and development depending on source due to soil nutrient variation among populations. Higher temperatures promoted increased germination percentages in South Carolina seeds, while Michigan and south Florida seed germination were higher at lower temperatures. Chilling seeds effectively increased germination in all populations, but seed germination in northern populations exceeded embryo viability in longer chilling treatments. This was reflected by the thicker testae required to protect seeds of northern populations form harsher winter climates. During a detailed timecourse seedling development study, Michigan seedlings allocated more biomass to corms, and developed corms faster than all other populations. Higher corm biomass allocation in northern populations correlated strongly with a shortened growing season. The rapid corm formation and biomass allocation in seedlings from more northern populations represented an adaptive response to a shorter growing season to increase survival. All populations required a chilling period to break corm dormancy, but longer chilling periods promoted faster and a higher percentage regrowth. Cutting corms also broke dormancy, but Michigan corms responded more rapidly compared to other populations. In conclusion, in vitro techniques were found to be appropriate to differentiate ecotypes by detecting their unique ecological growth strategies. Ecotypic differentiation influenced by growing season length, temperature, and soil nutrient availability is occurring in C. tuberosus.

ISBN: 9781109670929Subjects--Topical Terms:

1017825
Biology, Botany.

In vitro ecology of Calopogon tuberosus var. tuberosus: A new concept in species conservation.
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