東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Mathematical modeling of the benzeno...

Marshall Colon, Amy.

FindBook

Google Book

Amazon

博客來

Mathematical modeling of the benzenoid network in Petunia hybrida.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Mathematical modeling of the benzenoid network in Petunia hybrida./
作者:	Marshall Colon, Amy.
面頁冊數:	107 p.
附註:	Source: Dissertation Abstracts International, Volume: 70-11, Section: B, page: 6626.
Contained By:	Dissertation Abstracts International70-11B.
標題:	Agriculture, Horticulture. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3379667
ISBN:	9781109488227

Mathematical modeling of the benzenoid network in Petunia hybrida.
Marshall Colon, Amy.

Mathematical modeling of the benzenoid network in Petunia hybrida. - 107 p.

Source: Dissertation Abstracts International, Volume: 70-11, Section: B, page: 6626.

Thesis (Ph.D.)--Purdue University, 2009.

Emission of volatiles from plant floral and vegetative organs is important for reproductive success. Petunia flowers rhythmically emit a bouquet of benzenoid and phenylpropanoid compounds. The key benzenoid intermediate benzoic acid (BA) is believed to be synthesized from phenylalanine through the shortening of the propyl side-chain by two carbons. Limited knowledge is available about the enzymes involved in the chain-shortening reaction but it has been hypothesized that this reaction can occur via either a beta-oxidative or a non-beta-oxidative pathway. In vivo stable isotope labeling in combination with computer-assisted metabolic flux analysis (MFA) of the benzenoid network in petunia flowers revealed that both the beta-oxidative and non-beta-oxidative pathways contribute to the formation of benzenoids, and suggested that benzylbenzoate is an intermediate between L-Phe and BA. To test this hypothesis, transgenic petunia plants were generated in which the expression of benzyl alcohol/phenylethanol benzoyl transferase (BPBT) was down-regulated. Elimination of benzylbenzoate formation led to a reduction of endogenous BA and emitted methylbenzoate and increased benzylalcohol and benzylaldehyde emission, confirming the contribution of benzylbenzoate to BA formation. The combined approach of isotopic labeling, MFA, and targeted genetic manipulation was extended to develop a kinetic model capable of simulating whole network responses to different concentrations of supplied Phe in petunia flowers and capturing flux redistributions caused by genetic manipulations. A novel method for kinetic modeling of plant metabolism was employed to determine kinetic parameters for benzenoid network reactions. The model's ability to predict network response to genetic perturbations was validated by comparing simulated and observed redistributions of metabolite pool sizes and labeling data from transgenic petunia flowers. Model-determined kinetic parameters were used for metabolic control analysis, which described the distribution of control among network parameters on flux toward volatile emission as well as the differential regulation surrounding a key branch point at Phe. The modeling approach presented here is widely applicable to other plant metabolic networks. A valid kinetic model can simulate dozens of in silico metabolic engineering strategies for the study of plant metabolic networks and their regulation, which can ultimately save labor and expense and be a powerful predictive tool.

ISBN: 9781109488227Subjects--Topical Terms:

1017832
Agriculture, Horticulture.

Mathematical modeling of the benzenoid network in Petunia hybrida.
LDR:03593nam 2200313 4500 001 1397932
005 20110907152231.5
008 130515s2009 ||||||||||||||||| ||eng d
020 $a 9781109488227
035 $a (UMI)AAI3379667
035 $a AAI3379667
040 $a UMI $c UMI
100 1 $a Marshall Colon, Amy. $3 1676793
245 1 0 $a Mathematical modeling of the benzenoid network in Petunia hybrida.
300 $a 107 p.
500 $a Source: Dissertation Abstracts International, Volume: 70-11, Section: B, page: 6626.
500 $a Advisers: David Rhodes; Natalia Dudareva.
502 $a Thesis (Ph.D.)--Purdue University, 2009.
520 $a Emission of volatiles from plant floral and vegetative organs is important for reproductive success. Petunia flowers rhythmically emit a bouquet of benzenoid and phenylpropanoid compounds. The key benzenoid intermediate benzoic acid (BA) is believed to be synthesized from phenylalanine through the shortening of the propyl side-chain by two carbons. Limited knowledge is available about the enzymes involved in the chain-shortening reaction but it has been hypothesized that this reaction can occur via either a beta-oxidative or a non-beta-oxidative pathway. In vivo stable isotope labeling in combination with computer-assisted metabolic flux analysis (MFA) of the benzenoid network in petunia flowers revealed that both the beta-oxidative and non-beta-oxidative pathways contribute to the formation of benzenoids, and suggested that benzylbenzoate is an intermediate between L-Phe and BA. To test this hypothesis, transgenic petunia plants were generated in which the expression of benzyl alcohol/phenylethanol benzoyl transferase (BPBT) was down-regulated. Elimination of benzylbenzoate formation led to a reduction of endogenous BA and emitted methylbenzoate and increased benzylalcohol and benzylaldehyde emission, confirming the contribution of benzylbenzoate to BA formation. The combined approach of isotopic labeling, MFA, and targeted genetic manipulation was extended to develop a kinetic model capable of simulating whole network responses to different concentrations of supplied Phe in petunia flowers and capturing flux redistributions caused by genetic manipulations. A novel method for kinetic modeling of plant metabolism was employed to determine kinetic parameters for benzenoid network reactions. The model's ability to predict network response to genetic perturbations was validated by comparing simulated and observed redistributions of metabolite pool sizes and labeling data from transgenic petunia flowers. Model-determined kinetic parameters were used for metabolic control analysis, which described the distribution of control among network parameters on flux toward volatile emission as well as the differential regulation surrounding a key branch point at Phe. The modeling approach presented here is widely applicable to other plant metabolic networks. A valid kinetic model can simulate dozens of in silico metabolic engineering strategies for the study of plant metabolic networks and their regulation, which can ultimately save labor and expense and be a powerful predictive tool.
590 $a School code: 0183.
650 4 $a Agriculture, Horticulture. $3 1017832
690 $a 0471
710 2 $a Purdue University. $b Horticulture and Landscape Architecture. $3 1264121
773 0 $t Dissertation Abstracts International $g 70-11B.
790 1 0 $a Rhodes, David, $e advisor
790 1 0 $a Dudareva, Natalia, $e advisor
790 1 0 $a Chapple, Clint CS $e committee member
790 1 0 $a Goldsbrough, Peter B. $e committee member
790 1 0 $a Raghothama, Kashchandra G. $e committee member
790 $a 0183
791 $a Ph.D.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3379667