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Evaluation of the role of plant arch...

Ando, Kaori.

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Evaluation of the role of plant architecture and cucumber fruit development in Phytophthora capsici disease development.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Evaluation of the role of plant architecture and cucumber fruit development in Phytophthora capsici disease development./
作者:	Ando, Kaori.
面頁冊數:	146 p.
附註:	Source: Dissertation Abstracts International, Volume: 70-10, Section: B, page: 5911.
Contained By:	Dissertation Abstracts International70-10B.
標題:	Agriculture, Horticulture. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3381083
ISBN:	9781109413687

Evaluation of the role of plant architecture and cucumber fruit development in Phytophthora capsici disease development.
Ando, Kaori.

Evaluation of the role of plant architecture and cucumber fruit development in Phytophthora capsici disease development. - 146 p.

Source: Dissertation Abstracts International, Volume: 70-10, Section: B, page: 5911.

Thesis (Ph.D.)--Michigan State University, 2009.

Fruit rot caused by Phytophthora capsici Leonian is an increasingly serious disease affecting production of cucumber ( Cucumis sativus L.) and other cucurbit crops in many parts of the US. The absence of genetically resistant cultivars and rapid development of fungicide resistance makes it imperative to develop integrated disease management strategies. Cucumber fruit which come in direct contact with the soil-borne pathogen are usually located under the canopy where moist and warm conditions favor disease development. Screening a collection of 150 Plant Introductions (PI) revealed variation for an array of architectural traits. One of the compact lines (PI 308916), which had a tendency to hold young fruit off the ground, exhibited lower disease occurrence which was not due to genetic resistance, suggesting that architecture which allows less contact of fruit with the soil could be useful for P. capsici control for cucumber. In the course of screening for resistance sources among cucumber PIs, fruit age/size was shown to be a factor in susceptibility to infection by P. capsici. Inoculation of greenhouse-grown fruits of known ages showed that cucumber fruits were most susceptible to P. capsici when they were very young and rapidly elongating, but then developed age-related resistance (ARR) as they approach full length at 10-12 days post pollination (DPP). This was observed in both the greenhouse and field and for several genotypes. Testing of seven additional cucurbit crops, zucchini, summer squash, acorn squash, pumpkin, butternut squash, melon, and watermelon also showed ARR, but to varying degrees. In the field, infection primarily occurs at the blossom end. Inoculation of cucurbit fruits of various ages with P. capsici at the peduncle and blossom ends showed that as fruit age increased, the peduncle end became less susceptible sooner than the blossom end, suggesting a developmental gradient within the fruit influencing susceptibility for cucumber, zucchini, acorn, and butternut squash fruits. To understand the basis for ARR in the cucumber fruit-P. capsici interaction, and to increase understanding of early fruit development in cucumber, morphological and global gene expression analyses were performed. Morphological changes associated with cucumber fruit development were catalogued for hand pollinated greenhouse grown fruits at 0, 4, 8, 12, 16, 20, 26, and 32 DPP. These fruits were also collected to generate cDNA libraries for 454 pyrosequencing technology developed by RocheRTM. Young cucumber fruits show marked changes in fruit size, cell size, surface wax, chlorophyll content and patterns, wart formation, spine development, and placenta and seed development. 454 pyrosequencing analyses yielded 187,406 clean reads, of which 88% could be assembled into 13,879 contigs. The number of sequences per contig, which is reflective of transcript abundance, ranged from 2-5,167. BLAST analysis of the most highly represented transcripts against the nr protein sequence NCBI database, indicated high representation of genes associated with protein synthesis, flowers, fruits or seeds of other species, latex related proteins, lipid biosynthesis, cell expansion, defense, phloem transport, and photosynthesis. Results of 454 and qRT-PCR for selected genes were comparable, indicating that the 454 transcriptome sequencing can be used for analyzing relative gene expression during fruit development. These information will contribute to the understanding of biology of cucumber fruit development and possible relationship to age-related resistance.

ISBN: 9781109413687Subjects--Topical Terms:

1017832
Agriculture, Horticulture.

Evaluation of the role of plant architecture and cucumber fruit development in Phytophthora capsici disease development.
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