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Use of molecular and biochemical met...

Mohamed, Azza Hosni Ibrahim.

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Use of molecular and biochemical methods to determine citrus tristeza virus (CTV) viral components and resistance in candidate rootstocks to replace sour orange.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Use of molecular and biochemical methods to determine citrus tristeza virus (CTV) viral components and resistance in candidate rootstocks to replace sour orange./
Author:	Mohamed, Azza Hosni Ibrahim.
Description:	183 p.
Notes:	Source: Dissertation Abstracts International, Volume: 70-07, Section: B, page: 3846.
Contained By:	Dissertation Abstracts International70-07B.
Subject:	Agriculture, Horticulture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3367560
ISBN:	9781109280524

Use of molecular and biochemical methods to determine citrus tristeza virus (CTV) viral components and resistance in candidate rootstocks to replace sour orange.
Mohamed, Azza Hosni Ibrahim.

Use of molecular and biochemical methods to determine citrus tristeza virus (CTV) viral components and resistance in candidate rootstocks to replace sour orange. - 183 p.

Source: Dissertation Abstracts International, Volume: 70-07, Section: B, page: 3846.

Thesis (Ph.D.)--University of Florida, 2009.

Citrus tristeza virus (CTV) is the causal agent of the most destructive viral disease of citrus and has a big impact on citrus production all over the world. CTV is a phloem-limited virus that belongs to Closteroviridae family. The virus causes a wide range of symptoms depending on the isolate and the host. Sour orange (Citrus aurantium L.) has been a widely used rootstock for citrus because of its desirable qualities including resistance to phytophthora diseases and citrus blight, wide adaptation, and ability to produce good yields of high quality fruits. Unfortunately, citrus scions on sour orange rootstock are highly susceptible to quick decline (QD) disease caused by CTV. This has lead to the reduction of sour orange rootstock in Florida and in other citrus areas. The current rootstocks in Florida are primarily trifoliate hybrids which are not adapted to high pH, calcareous soils. Several new rootstocks have been developed in attempts to replace sour orange rootstock. Previous efforts to screen new hybrid rootstock candidates in the greenhouse for resistance to tristeza-QD have been confounded by another CTV disease called seedling yellows that affect only juvenile plants. The main objective of the present study was to develop a new assay that bypasses the seedling yellows effect. Seventy-two selections, including parental pummelos, pre-selected sour-orange-like pummelo-mandarin rootstock hybrids, and sour orange were top-worked onto 15-year old 'Hamlin' sweet orange trees known to carry the three CTV genotypes important in Florida (T30, T36 and VT). Virus infection was determined by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Over all, there was a significant difference in terms of shoot growth between the tested rootstock candidates and the sour orange that was stunted and showed strong disease symptoms. Movement of the various CTV genotypes from the 'Hamlin' interstock into the grafts was determined by molecular techniques including multiple molecular markers (MMM) analysis and heteroduplex mobility assay (HMA). Several CTV-induced quick decline resistant/tolerant selections, including some pummelo parents and new hybrids, were identified using quantitative real time PCR (qRT-PCR).

ISBN: 9781109280524Subjects--Topical Terms:

1017832
Agriculture, Horticulture.

Use of molecular and biochemical methods to determine citrus tristeza virus (CTV) viral components and resistance in candidate rootstocks to replace sour orange.
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520 $a Citrus tristeza virus (CTV) is the causal agent of the most destructive viral disease of citrus and has a big impact on citrus production all over the world. CTV is a phloem-limited virus that belongs to Closteroviridae family. The virus causes a wide range of symptoms depending on the isolate and the host. Sour orange (Citrus aurantium L.) has been a widely used rootstock for citrus because of its desirable qualities including resistance to phytophthora diseases and citrus blight, wide adaptation, and ability to produce good yields of high quality fruits. Unfortunately, citrus scions on sour orange rootstock are highly susceptible to quick decline (QD) disease caused by CTV. This has lead to the reduction of sour orange rootstock in Florida and in other citrus areas. The current rootstocks in Florida are primarily trifoliate hybrids which are not adapted to high pH, calcareous soils. Several new rootstocks have been developed in attempts to replace sour orange rootstock. Previous efforts to screen new hybrid rootstock candidates in the greenhouse for resistance to tristeza-QD have been confounded by another CTV disease called seedling yellows that affect only juvenile plants. The main objective of the present study was to develop a new assay that bypasses the seedling yellows effect. Seventy-two selections, including parental pummelos, pre-selected sour-orange-like pummelo-mandarin rootstock hybrids, and sour orange were top-worked onto 15-year old 'Hamlin' sweet orange trees known to carry the three CTV genotypes important in Florida (T30, T36 and VT). Virus infection was determined by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Over all, there was a significant difference in terms of shoot growth between the tested rootstock candidates and the sour orange that was stunted and showed strong disease symptoms. Movement of the various CTV genotypes from the 'Hamlin' interstock into the grafts was determined by molecular techniques including multiple molecular markers (MMM) analysis and heteroduplex mobility assay (HMA). Several CTV-induced quick decline resistant/tolerant selections, including some pummelo parents and new hybrids, were identified using quantitative real time PCR (qRT-PCR).
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