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Immunologic markers for assessment o...

Lee, Chia-Shan.

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Immunologic markers for assessment of Cetacean health.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Immunologic markers for assessment of Cetacean health./
作者:	Lee, Chia-Shan.
面頁冊數:	326 p.
附註:	Source: Dissertation Abstracts International, Volume: 72-05, Section: B, page: .
Contained By:	Dissertation Abstracts International72-05B.
標題:	Biology, Veterinary Science. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3444045
ISBN:	9781124508986

Immunologic markers for assessment of Cetacean health.
Lee, Chia-Shan.

Immunologic markers for assessment of Cetacean health. - 326 p.

Source: Dissertation Abstracts International, Volume: 72-05, Section: B, page: .

Thesis (Ph.D.)--University of California, Davis, 2010.

Peripheral blood leukocytes (PBLs) represent a clinically accessible representation of cells that play multiple roles in cellular and immunological interactions, such as innate and acquired immune responses. These leukocytes are key insult-associated triggers of the immune system and respond via induced transcription of select genes leading to generation of downstream soluble protein effectors and/or alterations in cell phenotype. Quantification of relative levels of such messengers and associated alterations in PBLs has been realized using quantitative real-time polymerase chain reaction (qPCR) and flow cytometry, in various cetacean species, including bottlenose dolphins and killer whales. Unfortunately, the characteristic of "relativity" in gene transcript analysis precludes establishment of universal references.

ISBN: 9781124508986Subjects--Topical Terms:

1021733
Biology, Veterinary Science.

Immunologic markers for assessment of Cetacean health.
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245 1 0 $a Immunologic markers for assessment of Cetacean health.
300 $a 326 p.
500 $a Source: Dissertation Abstracts International, Volume: 72-05, Section: B, page: .
500 $a Adviser: Jeffrey L. Stott.
502 $a Thesis (Ph.D.)--University of California, Davis, 2010.
520 $a Peripheral blood leukocytes (PBLs) represent a clinically accessible representation of cells that play multiple roles in cellular and immunological interactions, such as innate and acquired immune responses. These leukocytes are key insult-associated triggers of the immune system and respond via induced transcription of select genes leading to generation of downstream soluble protein effectors and/or alterations in cell phenotype. Quantification of relative levels of such messengers and associated alterations in PBLs has been realized using quantitative real-time polymerase chain reaction (qPCR) and flow cytometry, in various cetacean species, including bottlenose dolphins and killer whales. Unfortunately, the characteristic of "relativity" in gene transcript analysis precludes establishment of universal references.
520 $a A qPCR technique using Tursiops truncatus- and Orcinus orca-specific nucleotide primers was developed, validated and used to measure alterations in mRNA transcript levels encoding 11 immunologically relevant genes (Cox-2, TNFalpha, CD69, IL-2Ralpha, -4, -10, and -17, IFNalpha, IFNbeta, Mx1, and Fadd) associated with phagocytosis, pro- and anti-inflammatory responses, immunomodulation, T-cell activity, anti-viral responses and leukocyte apoptosis. Monoclonal antibodies, specific for leukocyte differentiation antigens, were used as markers for all leukocytes, myeloid cells, mononuclear cells and lymphocyte subsets (B, naive T and memory T). Baseline values for hematologic and blood biochemistry values and leukocyte subpopulations and their gene transcripts were established using multiple blood samples from domestically-maintained T. truncatus. Variability in select parameters was detected as a function of age, sex and geographic location. Negative correlations were identified between age and B lymphocytes, naive T lymphocytes and the percentage of polymorphonuclear leukocytes (PMNs) expressing high cell-surface densities of a cell adhesion maker referred to as "D". Taken together, the data suggested an association between PMN and lymphocyte activity.
520 $a Application of "normal" baselines values for leukocyte gene transcripts to the study of clinically-ill T. truncatus demonstrated both practicability and utility in distinguishing between groups of dolphins presenting with organ-specific disease. A retrospective and prospective study was conducted to compare leukocyte gene transcript profiles of pregnant and non-pregnant O. orca. Transcript levels of IL-4 and IL-17 were sometimes absent but did not correlate with pregnancy. No substantially aberrant IL-10 mRNA levels were noted in pregnant whales, suggesting an absence of an immunosuppressive system environment. In summary, the data demonstrate that qPCR and flow cytometry can serve as powerful tools in identifying cetaceans with specific organ system disorders and provide insight into concurrent immunological events throughout the clinical courses of the insult, thus assisting in clinical diagnoses, treatment strategy, and prognosis assessments.
590 $a School code: 0029.
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650 4 $a Health Sciences, Immunology. $3 1017716
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710 2 $a University of California, Davis. $b Comparative Pathology. $3 1676142
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