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Oxygen concentration microgradients ...

Park, Jaehyun.

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Oxygen concentration microgradients for cell culture.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Oxygen concentration microgradients for cell culture./
Author:	Park, Jaehyun.
Description:	107 p.
Notes:	Source: Dissertation Abstracts International, Volume: 71-09, Section: B, page: 5691.
Contained By:	Dissertation Abstracts International71-09B.
Subject:	Engineering, Biomedical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3413455
ISBN:	9781124141527

Oxygen concentration microgradients for cell culture.
Park, Jaehyun.

Oxygen concentration microgradients for cell culture. - 107 p.

Source: Dissertation Abstracts International, Volume: 71-09, Section: B, page: 5691.

Thesis (Ph.D.)--University of California, Berkeley, 2010.

There is a growing need for technology that can control microscale oxygen gradients onto a tissue or culture sample in vitro. This dissertation introduces the oxygen microgradient chip (OMA), which employs electrolysis to generate oxygen microgradients within cell culture without forming bubbles. Dissolved oxygen generated at noble microelectrodes patterned on a chip surface diffuses through a gas-permeable silicone membrane and is dosed into cell culture. The amount of generated oxygen is directly proportional to a current flowing across the electrodes and thus can be controlled with high precision. By real-time modulation of the current, spatial profile of the oxygen concentration microgradient can be modified during an experiment. Distinct microgradients generated by different electrodes can be superimposed to pattern arbitrary and multi-dimensional oxygen concentration profiles with microscale resolution. Design of the OMA is based on simulation results of diffusion and physical considerations. Microfabrication, assembly process, and improvements of the early OMA are presented. Use of an oxygen-sensitive fluorophore film verifies that the measured microgradients in OMA match simulation results. Generation of reactive oxygen species during the electrolysis is also considered and quantified. This technology enables the biological study of gradient-related effects. Three different, well-known biological models are introduced to demonstrate how the microtechnology enables new types of experiments. Lastly, we explore the effect of oxygen gradients on muscle progenitor cells having half-deficiency of superoxide dismutase-1 (SOD1) enzyme. Another type of oxygen gradient chip that utilizes atmospheric oxygen is developed and used in this experiment. Myotube formation of the SOD1 heterozygote myoblasts decreased significantly compared to that of wild-type myoblasts, indicating that the oxygen stress on SOD1 heterozygote myoblasts induces defective cellular function on differentiation.

ISBN: 9781124141527Subjects--Topical Terms:

1017684
Engineering, Biomedical.

Oxygen concentration microgradients for cell culture.
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020 $a 9781124141527
035 $a (UMI)AAI3413455
035 $a AAI3413455
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100 1 $a Park, Jaehyun. $3 1672249
245 1 0 $a Oxygen concentration microgradients for cell culture.
300 $a 107 p.
500 $a Source: Dissertation Abstracts International, Volume: 71-09, Section: B, page: 5691.
500 $a Adviser: Michel M. Maharbiz.
502 $a Thesis (Ph.D.)--University of California, Berkeley, 2010.
520 $a There is a growing need for technology that can control microscale oxygen gradients onto a tissue or culture sample in vitro. This dissertation introduces the oxygen microgradient chip (OMA), which employs electrolysis to generate oxygen microgradients within cell culture without forming bubbles. Dissolved oxygen generated at noble microelectrodes patterned on a chip surface diffuses through a gas-permeable silicone membrane and is dosed into cell culture. The amount of generated oxygen is directly proportional to a current flowing across the electrodes and thus can be controlled with high precision. By real-time modulation of the current, spatial profile of the oxygen concentration microgradient can be modified during an experiment. Distinct microgradients generated by different electrodes can be superimposed to pattern arbitrary and multi-dimensional oxygen concentration profiles with microscale resolution. Design of the OMA is based on simulation results of diffusion and physical considerations. Microfabrication, assembly process, and improvements of the early OMA are presented. Use of an oxygen-sensitive fluorophore film verifies that the measured microgradients in OMA match simulation results. Generation of reactive oxygen species during the electrolysis is also considered and quantified. This technology enables the biological study of gradient-related effects. Three different, well-known biological models are introduced to demonstrate how the microtechnology enables new types of experiments. Lastly, we explore the effect of oxygen gradients on muscle progenitor cells having half-deficiency of superoxide dismutase-1 (SOD1) enzyme. Another type of oxygen gradient chip that utilizes atmospheric oxygen is developed and used in this experiment. Myotube formation of the SOD1 heterozygote myoblasts decreased significantly compared to that of wild-type myoblasts, indicating that the oxygen stress on SOD1 heterozygote myoblasts induces defective cellular function on differentiation.
590 $a School code: 0028.
650 4 $a Engineering, Biomedical. $3 1017684
650 4 $a Engineering, Electronics and Electrical. $3 626636
690 $a 0541
690 $a 0544
710 2 $a University of California, Berkeley. $b Electrical Engineering & Computer Sciences. $3 1671057
773 0 $t Dissertation Abstracts International $g 71-09B.
790 1 0 $a Maharbiz, Michel M., $e advisor
790 1 0 $a Wu, Ming C. $e committee member
790 1 0 $a Herr, Amy E. $e committee member
790 $a 0028
791 $a Ph.D.
792 $a 2010
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3413455