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Use of ionomics to identify genes th...

Morrissey, Joe.

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Use of ionomics to identify genes that play a role in Arabidopsis metal homeostasis.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Use of ionomics to identify genes that play a role in Arabidopsis metal homeostasis./
作者:	Morrissey, Joe.
面頁冊數:	313 p.
附註:	Source: Dissertation Abstracts International, Volume: 71-12, Section: B, page: .
Contained By:	Dissertation Abstracts International71-12B.
標題:	Biology, Botany. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3427645
ISBN:	9781124322063

Use of ionomics to identify genes that play a role in Arabidopsis metal homeostasis.
Morrissey, Joe.

Use of ionomics to identify genes that play a role in Arabidopsis metal homeostasis. - 313 p.

Source: Dissertation Abstracts International, Volume: 71-12, Section: B, page: .

Thesis (Ph.D.)--Dartmouth College, 2011.

Understanding trace element homeostasis in plants has a direct impact on global health. Much of the developing world suffers from nutrient deficiencies, and subsists on a plant based diet often lacking bioavailable nutrients like iron and zinc. At the same time, industrial pollution of farmland has contaminated soils with toxic metals like cadmium, nickel, and cobalt. Elucidating the mechanisms of uptake and availability of trace elements could speed the breeding of more nutritious crops that exclude the uptake of toxic metals. Our approach to identifying the genes that control these mechanisms is ionomics, which utilizes high-throughput elemental analysis of mutant lines and ecotypes to identify genes that control metal homeostasis. From several ionomic screens we have identified and characterized genes that play a role in nickel tolerance, iron accumulation in seeds, and natural variation of cobalt accumulation in roots and shoots.

ISBN: 9781124322063Subjects--Topical Terms:

1017825
Biology, Botany.

Use of ionomics to identify genes that play a role in Arabidopsis metal homeostasis.
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245 1 0 $a Use of ionomics to identify genes that play a role in Arabidopsis metal homeostasis.
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500 $a Source: Dissertation Abstracts International, Volume: 71-12, Section: B, page: .
500 $a Adviser: Mary Lou Guerinot.
502 $a Thesis (Ph.D.)--Dartmouth College, 2011.
520 $a Understanding trace element homeostasis in plants has a direct impact on global health. Much of the developing world suffers from nutrient deficiencies, and subsists on a plant based diet often lacking bioavailable nutrients like iron and zinc. At the same time, industrial pollution of farmland has contaminated soils with toxic metals like cadmium, nickel, and cobalt. Elucidating the mechanisms of uptake and availability of trace elements could speed the breeding of more nutritious crops that exclude the uptake of toxic metals. Our approach to identifying the genes that control these mechanisms is ionomics, which utilizes high-throughput elemental analysis of mutant lines and ecotypes to identify genes that control metal homeostasis. From several ionomic screens we have identified and characterized genes that play a role in nickel tolerance, iron accumulation in seeds, and natural variation of cobalt accumulation in roots and shoots.
520 $a By analyzing Arabidopsis ecotypes for variation in cobalt accumulation, we found that several cobalt accumulating ecotypes contained mutations in the FPN2 gene. Arabidopsis FPN2 and its paralog FPN1 are related to animal ferroportin protein, the sole iron efflux transporter in vertebrates. Our characterization of the Arabidopsis ferroportins revealed that they efflux both iron and cobalt, playing a role in iron homeostasis, in addition to controlling cobalt localization and tolerance.
520 $a From ionomic screens for mutations that alter metal accumulation in shoots and seeds of Arabidopsis, we identified ionomic phenotypes controlled by genes previously unknown to play a role in metal homeostasis: the loss of SQD2 of the sulfolipid biosynthesis pathway results in nickel tolerance; the loss of NDUFA9 of mitochondrial complex I results in decreased iron concentration in seed; and CDC73 of the Paf1 complex is essential for maintaining metal homeostasis in the shoot. Additionally, two fast neutron Arabidopsis mutants with altered iron accumulation in the seeds were identified. One mutant is able to thrive and accumulate seed iron under iron limiting conditions, while the other mutant shows increased sensitivity to iron limitation and cannot set seed when grown on alkaline soil.
520 $a Ultimately, these results demonstrate that ionomics is an effective approach to identify genes that control metal homeostasis.
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