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Tissue-specific gene expression of t...

Liu, Tianzhen.

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Tissue-specific gene expression of two class III Arabidopsis peroxidases under aluminum stress.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Tissue-specific gene expression of two class III Arabidopsis peroxidases under aluminum stress./
作者:	Liu, Tianzhen.
面頁冊數:	161 p.
附註:	Source: Masters Abstracts International, Volume: 48-05, page: 2825.
Contained By:	Masters Abstracts International48-05.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MR60591
ISBN:	9780494605912

Tissue-specific gene expression of two class III Arabidopsis peroxidases under aluminum stress.
Liu, Tianzhen.

Tissue-specific gene expression of two class III Arabidopsis peroxidases under aluminum stress. - 161 p.

Source: Masters Abstracts International, Volume: 48-05, page: 2825.

Thesis (M.Sc.)--University of Alberta (Canada), 2010.

Class III peroxidases have been identified as secreted proteins involved in plant defense, auxin metabolism, cell wall modification, and regulation of reactive oxygen species. Transcriptome analysis of Arabidopsis has identified many class III peroxidases that respond to aluminum stress. The large number and versatile function of peroxidases make functional characterization of individual peroxidases a challenging task. However, the use of promoter::GUS reporter fusions can help to elucidate the expression pattern of individual peroxidases. To investigate the expression of PER22 and PER73 under aluminum stress in Arabidopsis, single-copy, homozygous, transgenic plants harbouring promoter::GUS reporter fusions were generated through self-pollination and southern blot analyses. Histochemical GUS staining of transgenic lines revealed trichome-specific and vascular-specific expression patterns for PER22 and PER73, respectively. The temporal and spatial expression of PER22 and PER73 suggest that they might be involved in pathogen defense and/or lignification. Further experiments will help define these peroxidase functions under aluminum stress.

ISBN: 9780494605912Subjects--Topical Terms:

1017719
Biology, Molecular.

Tissue-specific gene expression of two class III Arabidopsis peroxidases under aluminum stress.
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520 $a Class III peroxidases have been identified as secreted proteins involved in plant defense, auxin metabolism, cell wall modification, and regulation of reactive oxygen species. Transcriptome analysis of Arabidopsis has identified many class III peroxidases that respond to aluminum stress. The large number and versatile function of peroxidases make functional characterization of individual peroxidases a challenging task. However, the use of promoter::GUS reporter fusions can help to elucidate the expression pattern of individual peroxidases. To investigate the expression of PER22 and PER73 under aluminum stress in Arabidopsis, single-copy, homozygous, transgenic plants harbouring promoter::GUS reporter fusions were generated through self-pollination and southern blot analyses. Histochemical GUS staining of transgenic lines revealed trichome-specific and vascular-specific expression patterns for PER22 and PER73, respectively. The temporal and spatial expression of PER22 and PER73 suggest that they might be involved in pathogen defense and/or lignification. Further experiments will help define these peroxidase functions under aluminum stress.
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